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Methylene Blue Dyes inhibitor

Name: Methylene Blue
Brand: Selleck Chemicals
SKU: S4535
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S4535

Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015) is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.

Chemical Structure

Molecular Weight: 319.85

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Related Targets	CXCR Hedgehog/Smoothened PKA Adrenergic Receptor AChR 5-HT Receptor Histamine Receptor Dopamine Receptor Ras KRas
Other Dyes Inhibitors	CFSE Propidium Iodide D-Luciferin D-Luciferin sodium salt Crystal Violet Hematoxylin DFO WST-8 Thiazolyl Blue Dihydroethidium

Solubility

In vitro
Batch:

DMSO : 64 mg/mL (200.09 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 64 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.15mg/ml (9.85mM)	Taking the 1 mL working solution as an example, add 50 μL of 63 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.315mg/ml (0.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	319.85	Formula	C₁₆H₁₈ClN₃S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	61-73-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015			Smiles	CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]

Mechanism of Action

Targets/IC₅₀/Ki	guanylate cyclase Tau aggregation 1.9 μM
In vitro	MB may inhibit the action of nitric oxide synthase itself and could also inactivate nitric oxide directly, possibly through the generation of superoxide anions. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, MB has also been shown to directly activate calcium-dependent potassium channels and to enhance release of noradrenaline from intracellular stores.
In vivo	Methylene Blue acts by inhibiting guanylate cyclase, thus decreasing C-GMP and vascular smooth muscle relaxation. When injected intravenously, MB selectively accumulates in parathyroid glands, thereby facilitating the identification of these structures during surgery. Pharmacokinetic studies in rodents revealed that intravascular administration of MB leads to a rapid and extensive accumulation of this drug in the central nervous system (CNS).
References	[1] https://pubmed.ncbi.nlm.nih.gov/18362601/ [2] https://pubmed.ncbi.nlm.nih.gov/25958115/ [3] https://pubmed.ncbi.nlm.nih.gov/10439772/ [4] https://pubmed.ncbi.nlm.nih.gov/21547182/

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