Methylene Blue

For research use only.

Catalog No.S4535 Synonyms: Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015

1 publication

Methylene Blue Chemical Structure

CAS No. 61-73-4

Methylene Blue is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.

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Selleck's Methylene Blue has been cited by 1 publication

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Biological Activity

Description Methylene Blue is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.
Targets
guanylate cyclase [3] Tau aggregation [2]
1.9 μM
In vitro

MB may inhibit the action of nitric oxide synthase itself and could also inactivate nitric oxide directly, possibly through the generation of superoxide anions. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, MB has also been shown to directly activate calcium-dependent potassium channels and to enhance release of noradrenaline from intracellular stores[1].

In vivo Methylene Blue acts by inhibiting guanylate cyclase, thus decreasing C-GMP and vascular smooth muscle relaxation. When injected intravenously, MB selectively accumulates in parathyroid glands, thereby facilitating the identification of these structures during surgery. Pharmacokinetic studies in rodents revealed that intravascular administration of MB leads to a rapid and extensive accumulation of this drug in the central nervous system (CNS)[1].

Protocol

Cell Research:

[1]

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  • Cell lines: Immature neuroblasts
  • Concentrations: 10 μM
  • Incubation Time: 2 h
  • Method:

    single cell culture model where immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are usd for investigations. Cultures are exposed at the sixth day in vitro to MB for 2 h and then we assess the survival and dendritic arbor architecture of these cells 48 h after this treatment paradigm.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Sprague- Dawley male rats
  • Dosages: 5 and 50 mg/kg
  • Administration: intravascular injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 63 mg/mL warmed (196.96 mM)
Water 63 mg/mL warmed (196.96 mM)
Ethanol ''10 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 319.85
Formula

C16H18ClN3S

CAS No. 61-73-4
Storage powder
in solvent
Synonyms Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015
Smiles CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.[Cl-]

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID