Methylene Blue

Catalog No.S4535 Batch:S453501

Technical Data

Formula

C₁₆H₁₈ClN₃S

Molecular Weight

319.85

CAS No.

61-73-4

Solubility (25°C)*

In vitro

DMSO

63 mg/mL (196.96 mM)

Water

63 mg/mL (196.96 mM)

Ethanol

10 mg/mL (31.26 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution	saline	63.000mg/ml (196.97mM)	Taking the 1 mL working solution as an example, add 63 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.15mg/ml (9.85mM)	Taking the 1 mL working solution as an example, add 50 μL of 63 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.315mg/ml (0.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015) is used as a dye in chromoendoscopy. It Inhibits tau filament formation with IC50 of 1.9μM. Also it inhibits soluble guanylyl cyclase.

Targets

guanylate cyclase ^[3]	Tau aggregation ^[2]
	1.9 μM

In vitro

MB may inhibit the action of nitric oxide synthase itself and could also inactivate nitric oxide directly, possibly through the generation of superoxide anions. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, MB has also been shown to directly activate calcium-dependent potassium channels and to enhance release of noradrenaline from intracellular stores^[1].

In vivo

Methylene Blue acts by inhibiting guanylate cyclase, thus decreasing C-GMP and vascular smooth muscle relaxation. When injected intravenously, MB selectively accumulates in parathyroid glands, thereby facilitating the identification of these structures during surgery. Pharmacokinetic studies in rodents revealed that intravascular administration of MB leads to a rapid and extensive accumulation of this drug in the central nervous system (CNS)^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Immature neuroblasts Concentrations 10 μM Incubation Time 2 h Method single cell culture model where immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are usd for investigations. Cultures are exposed at the sixth day in vitro to MB for 2 h and then we assess the survival and dendritic arbor architecture of these cells 48 h after this treatment paradigm.
Animal Study:^{^[1]}	Animal Models Sprague- Dawley male rats Dosages 5 and 50 mg/kg Administration intravascular injection

Cell Assay:^{^[1]}

Cell lines
Immature neuroblasts
Concentrations
10 μM
Incubation Time
2 h
Method
single cell culture model where immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are usd for investigations. Cultures are exposed at the sixth day in vitro to MB for 2 h and then we assess the survival and dendritic arbor architecture of these cells 48 h after this treatment paradigm.

Animal Study:^{^[1]}

Animal Models
Sprague- Dawley male rats
Dosages
5 and 50 mg/kg
Administration
intravascular injection

References

[4] Ginimuge PR, et al. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520.

+ Expand

Selleck's Methylene Blue has been cited by 2 publications

Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids [ Cell Res, 2023, 10.1038/s41422-023-00846-8]	PubMed: 37460804
Reversal of Infected Host Gene Expression Identifies Repurposed Drug Candidates for COVID-19 [ bioRxiv, 2020, 2020/9/20.4.7.30734]	PubMed: 32511305

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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