Home ER stress & UPR ROS activator MCB-613

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MCB-613 ROS activator

Cat.No.S7913

MCB-613 is a potent steroid receptor coactivator (SRC) stimulator.
MCB-613 ROS activator Chemical Structure

Chemical Structure

Molecular Weight: 304.39

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Quality Control

Batch: S791301 DMSO]60 mg/mL]false]Ethanol]60 mg/mL]false]Water]Insoluble]false Purity: 99.97%
99.97

Solubility

In vitro
Batch:

DMSO : 60 mg/mL (197.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble

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Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 304.39 Formula

C20H20N2O

 Storage (From the date of receipt)
CAS No. 1162656-22-5 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CCC1CC(=CC2=CN=CC=C2)C(=O)C(=CC3=CN=CC=C3)C1

Mechanism of Action

Targets/IC50/Ki
SRC
In vitro
MCB-613 selectively and reversibly binds to the RID of SRC-3, and selectively kills cancer cells including MCF-7 (breast), PC-3 (prostate), H1299 (lung), and HepG2 (liver) cells, without toxicity to mouse primary hepatocytes and mouse embryonic fibroblasts (MEFs). This compound also increases SRCs’ interactions with other coactivators and markedly induces ER stress coupled to the generation of reactive oxygen species (ROS).
Kinase Assay
Luciferase assays
After various compound treatments, cells are lysed in luciferase lysis buffer and assayed for luciferase activity using the ONE-Glo luciferase assay system. All luciferase activities are normalized to protein concentration determined by Bradford assay.
In vivo
In an MCF-7 breast cancer mouse xenograft model, MCB-613 (20 mg/kg, i.p.) significantly and dramatically inhibits the growth of the tumor while causing no obvious animal toxicity and body weight less.
References

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