Linderane

Catalog No.S9229

For research use only.

Linderane, isolated from Lindera strychnifolia vill., is an indirect PDE3 activator and possesses multiple biological effects, including superoxide anion radical-scavenging and antioxidative activity and protective activity against gastritis, gastric ulcers and backache.

Linderane Chemical Structure

CAS No. 13476-25-0

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Biological Activity

Description Linderane, isolated from Lindera strychnifolia vill., is an indirect PDE3 activator and possesses multiple biological effects, including superoxide anion radical-scavenging and antioxidative activity and protective activity against gastritis, gastric ulcers and backache.
Targets
PDE3 [1]
()
In vitro

Linderane inhibits gluconeogenesis by activating hepatic PDE3 in rat primary hepatocytes. It reduces phosphoenolpyruvate carboxykinase (Pck1) and glucose-6-phosphatase (G6pc) gene expression, and decreases intracellular cAMP concentration and CREB phosphorylation in rat primary hepatocytes under both basal and forskolin-stimulated conditions. Linderane also increases total PDE and PDE3 activity but not PDE4 activity in rat primary hepatocytes. Linderane indirectly activated PDE3 through extracellular regulated protein kinase 1/2 (ERK1/2) and signal transducer and activator of transcription 3 (STAT3) activation[1].

In vivo Linderane improved glucose and lipid metabolism after chronic oral administration in ob/ob mice[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: rat primary hepatocytes
  • Concentrations: 2.5, 5, 10, 20 μM
  • Incubation Time: 1.5 h
  • Method:

    Rat primary hepatocytes are incubated in serum-free, glucose-free Dulbecco's modified Eagle's medium (DMEM) supplemented with metformin (500 μM) or different doses of linderane for 1.5 h, followed by 4 h of incubation in the presence or absence of gluconeogenic substrates (2 mM sodium pyruvate, plus 20 mM sodium lactate) with or without the stimulation of forskolin (20 μM). Medium is collected to determine glucose production.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: C57BL/6J mice (male, 8–9 weeks)
  • Dosages: 50 mg/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 52 mg/mL
(199.77 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 260.29
Formula

C15H16O4

CAS No. 13476-25-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CCCC23C(O2)C(C4=C(C1)OC=C4C)OC3=O

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