Leonurine

Catalog No.S3890

For research use only.

Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.

Leonurine Chemical Structure

CAS No. 24697-74-3

Purity & Quality Control

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Biological Activity

Description Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.
In vitro

Leonurine inhibits U937 cells adhesion to TNF-α-activated HUVEC in a concentration dependent manner. Treatment with leonurine blocks TNF-α-induced mRNA and protein expression of adhesion molecules (intercellular adhesion molecule-1 and vascular cell adhesion molecule-1), cyclooxygenase-2, and monocyte chemoattractant protein-1 in endothelial cells. In addition, leonurine attenuates TNF-α-induced intracellular ROS production in HUVEC. Furthermore, leonurine suppresses the TNF-α-activated p38 phosphorylation and IκBα degradation. Subsequently reduced NF-κB p65 phosphorylation, nuclear translocation, and DNA-binding activity are also observed[1]. Leonurine attenuates DOX-induced apoptosis in H9c2 cell by increasing anti-oxidant, anti-apoptotic ability and protecting mitochondrial function[4].

In vivo Leonurine attenuates hyperalgesia in mice with induced adenomyosis via down-regulating expressions of p-P65, COX-2, and OTR, and could be beneficial for treating adenomyosis[2]. Leonurine exerts antidepressant-like effects, which may be mediated, at least in part, by improving monoamine neurotransmitters and inhibiting neuroinflammation. Leonurine possesses neuroprotective effects in animal models of ischemic stroke, parkinson's disease, and alzheimer's disease. Leonurine treatment significantly rescues behavioral deficit of animals, promotes neuronal survival, and inhibits inflammation[3].

Protocol (from reference)

Cell Research:

[4]

  • Cell lines: Rat embryonic ventricularmyocardial H9c2 cell line
  • Concentrations: 10 μM
  • Incubation Time: 2 h
  • Method:

    Cells are using for Hoechst staining after treatement. DOX treatment: cells are exposed to 1 μM DOX for 24 h; Leo treatment: cells are pre-treated with 10 μM Leo for 2 h before exposure to DOX.

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: Male C57BL/6 (8–10 weeks) mice
  • Dosages: 30 and 60 mg/kg
  • Administration: intragastric administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 62 mg/mL
(199.14 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 311.33
Formula

C14H21N3O5

CAS No. 24697-74-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC(=CC(=C1O)OC)C(=O)OCCCCN=C(N)N

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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