Home Autophagy Autophagy chemical Leonurine For research use only.

Leonurine

Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.

Leonurine Chemical Structure

Leonurine Chemical Structure

CAS: 24697-74-3

Purity & Quality Control

Batch: S389001 DMSO] Insoluble] false] ] ] false] ] ] false Purity: 99.74%
99.74

Leonurine Related Products

Signaling Pathway

Autophagy Signaling Pathways

Autophagy Signaling Pathway

Choose Selective Autophagy Inhibitors

Biological Activity

Description Leonurine, an active alkaloid extracted from Traditional Chinese Medicine Herba leonuri, exerts several biological effects, such as antidiabetic, cardiovascular, and bovine mastitis protection.
In vitro
In vitro Leonurine inhibits U937 cells adhesion to TNF-α-activated HUVEC in a concentration dependent manner. Treatment with leonurine blocks TNF-α-induced mRNA and protein expression of adhesion molecules (intercellular adhesion molecule-1 and vascular cell adhesion molecule-1), cyclooxygenase-2, and monocyte chemoattractant protein-1 in endothelial cells. In addition, leonurine attenuates TNF-α-induced intracellular ROS production in HUVEC. Furthermore, leonurine suppresses the TNF-α-activated p38 phosphorylation and IκBα degradation. Subsequently reduced NF-κB p65 phosphorylation, nuclear translocation, and DNA-binding activity are also observed[1]. Leonurine attenuates DOX-induced apoptosis in H9c2 cell by increasing anti-oxidant, anti-apoptotic ability and protecting mitochondrial function[4].
Cell Research Cell lines Rat embryonic ventricularmyocardial H9c2 cell line
Concentrations 10 μM
Incubation Time 2 h
Method

Cells are using for Hoechst staining after treatement. DOX treatment: cells are exposed to 1 μM DOX for 24 h; Leo treatment: cells are pre-treated with 10 μM Leo for 2 h before exposure to DOX.
In Vivo
In vivo Leonurine attenuates hyperalgesia in mice with induced adenomyosis via down-regulating expressions of p-P65, COX-2, and OTR, and could be beneficial for treating adenomyosis[2]. Leonurine exerts antidepressant-like effects, which may be mediated, at least in part, by improving monoamine neurotransmitters and inhibiting neuroinflammation. Leonurine possesses neuroprotective effects in animal models of ischemic stroke, parkinson's disease, and alzheimer's disease. Leonurine treatment significantly rescues behavioral deficit of animals, promotes neuronal survival, and inhibits inflammation[3].
Animal Research Animal Models Male C57BL/6 (8–10 weeks) mice
Dosages 30 and 60 mg/kg
Administration intragastric administration

Chemical Information & Solubility

Molecular Weight 311.33 Formula

C14H21N3O5
CAS No. 24697-74-3 SDF Download Leonurine SDF
Smiles COC1=CC(=CC(=C1O)OC)C(=O)OCCCCN=C(N)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Leonurine | Leonurine supplier | purchase Leonurine | Leonurine cost | Leonurine manufacturer | order Leonurine | Leonurine distributor