L-SelenoMethionine

Catalog No.S3973

For research use only.

L-SelenoMethionine (SeMet), a naturally occurring amino acid, is a major food-form of selenium. It has been used as a supplement, and exhibits some important functions like cancer prevention and antioxidative defense.

L-SelenoMethionine Chemical Structure

CAS No. 3211-76-5

Purity & Quality Control

Choose Selective ROS Inhibitors

Biological Activity

Description L-SelenoMethionine (SeMet), a naturally occurring amino acid, is a major food-form of selenium. It has been used as a supplement, and exhibits some important functions like cancer prevention and antioxidative defense.
In vitro

L-SelenoMethionine (SeMet) has also been shown to contribute to the mitigation of radiation-induced oxidative stress. L-SelenoMethionine has the ability to selectively induce apoptosis and growth arrest in cancerous, but not normal, human prostate cells. Mechanistically, SeMet treatment is shown to promote growth inhibition in human colon cancer cells through the phosphorylation of histone H3 via the activation of MAPK extracellular regulated kinase (ERK) and subsequent phosphorylation of the ribosomal S6 kinase (RSK)[1].

In vivo L-SelenoMethionine (SeMet) increases survival time of NK/Ly lymphoma bearing animals treated with doxorubicin. L-SelenoMethionine (SeMet) can effectively protect the hematopoietic system and liver of tumor-bearing animals with NK/Ly lymphoma from the side effects of anticancer drug doxorubicin, which is revealed by normalization of blood indexes, animal weight, and ALT/AST activity[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Human thyroid epithelial cells (HTori-3)
  • Concentrations: 5 μM
  • Incubation Time: 24 h
  • Method: Human thyroid epithelial cells (HTori-3) are maintained in Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 1% glutamine and 10% FBS (growth medium). Twenty-four hours prior to irradiation with iron ions, fresh medium with or without 5 μM SeM is added. At the time of radiation exposure, the cells are approximately 80% confluent.
Animal Research:[2]
  • Animal Models: NL/Ly lymphoma-bearing mice
  • Dosages: 60 μg/kg, cumulative dose 600 μg/kg
  • Administration: p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 196.11
Formula

C5H11NO2Se

CAS No. 3211-76-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C[Se]CCC(C(=O)O)N

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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