Kojic acid

For research use only.

Catalog No.S5174

1 publication

Kojic acid Chemical Structure

CAS No. 501-30-4

Kojic acid is a fungal metabolite that inhibits tyrosinase with an IC50 value of 30.6 µM for mushroom tyrosinase. It is a synthetic intermediate used for production of food additives.

Selleck's Kojic acid has been cited by 1 publication

Purity & Quality Control

Choose Selective Tyrosinase Inhibitors

Biological Activity

Description Kojic acid is a fungal metabolite that inhibits tyrosinase with an IC50 value of 30.6 µM for mushroom tyrosinase. It is a synthetic intermediate used for production of food additives.
Targets
Tyrosinase [2]
()
0.28 mM
In vitro

Kojic acid (KA) acts as a good chelator of transition metal ions such as Cu2+ and Fe3+ and a scavenger of free radicals. Kojic acid shows a competitive inhibitory effect on the monophenolase activity and a mixed inhibitory effect on the diphenolase activity of mushroom tyrosinase[1]. Kojic acid could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity[3].

In vivo Kojic acid exerts its radioprotective effects on lethal or sub-lethal irradiation induced damages in mice[3].

Protocol

Cell Research:[1]
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  • Cell lines: CHO cells
  • Concentrations: 0, 0.1, 1, 10, 100, 1000, 1500, 2500 μg/ml
  • Incubation Time: 72 h
  • Method: CHO cells are incubated in RPMI 1640 medium containing 10% (v/v) fetal bovine serum, and maintained at 37°C in a 5% CO2 incubator. For cytotoxicity assay, CHO cells are seeded in 96-well plates at a density of 2500 cells per well in 180 μl medium. Then, 20 μl of Kojic acid stock solution are added to each well to make sure Kojic acid at the final concentrations of 0, 0.1, 1, 10, 100, 1000, 1500, 2500 μg/ml. After cultured for 72 h, each well of the cells is added with 20 μl MTT (5 mg/mL), which is dissolved in sterile Phosphate Buffered Saline (pH 7.4) and incubated for 4 h at 37°C. Then thesupernatant is discarded and 200 μl DMSO is added to each well. The mixture is sufficiently oscillated in the dark place for 15 min. The optical density values of wells are measured at 492 nm.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 28 mg/mL (197.03 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 142.11
Formula

C6H6O4

CAS No. 501-30-4
Storage powder
in solvent
Synonyms N/A
Smiles C1=C(OC=C(C1=O)O)CO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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% DMSO % % Tween 80 % ddH2O
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID