Echinacoside

For research use only.

Catalog No.S3783

1 publication

Echinacoside Chemical Structure

CAS No. 82854-37-3

Echinacoside, a natural polyphenolic compound, has various biological activities, such as antioxidative, anti-inflammatory, neuroprotective, hepatoprotective, nitric oxide radical-scavenging and vasodilative actions. Echinacoside, one of the phenylethanoids isolated from the stems of Cistanche salsa, elicits neuroprotection by activating Trk receptors and their downstream signal pathways. Echinacoside also has a remarkable antiosteoporotic activity.

Selleck's Echinacoside has been cited by 1 publication

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Biological Activity

Description Echinacoside, a natural polyphenolic compound, has various biological activities, such as antioxidative, anti-inflammatory, neuroprotective, hepatoprotective, nitric oxide radical-scavenging and vasodilative actions. Echinacoside, one of the phenylethanoids isolated from the stems of Cistanche salsa, elicits neuroprotection by activating Trk receptors and their downstream signal pathways. Echinacoside also has a remarkable antiosteoporotic activity.
In vitro

Echinacoside behaves in vitro as an anti-oxidant and a potent free radical scavenger. It has been shown to rescue SH-SY5Y neuronal cells from TNF-alpha-induced apoptosis. Echinacoside significantly attenuates glutamate-induced neurotoxicity and exhibits neuroprotective activities in vitro[1]. Echinacoside has significant neuroprotective effects on TNFα-induced apoptosis in SHSY5Y neuronal cells by maintaining mitochondrial function, decreasing the generation of reactive oxygen species, increasing the expression of the antiapoptotic protein Bcl-2 and inhibiting caspase-3 activity through an antioxidation mechanism[2].

In vivo Echinacoside has neuroprotective effects in the mouse MPTP model of Parkinson's disease. Treatment with echinacoside improves motor behavior and attenuated the depletion of striatal dopamine, DOPAC and HVA in MPTP-lesioned mice[1].

Protocol

Cell Research:[2]
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  • Cell lines: SHSY5Y cells
  • Concentrations: 1, 10 or 100 μg/ml
  • Incubation Time: 36 h
  • Method: Cell viability is determined by use of an MTT assay. Cells are seeded in 96-well plates at 1×104 cells per well and grown to 70% confluence in culture medium. The medium is replaced by medium containing various concentrations of echinacoside (1, 10 or 100 μg/ml) and/or 100 ng/ml TNFα. A total of 5 mg/ml MTT is added to each well after 36 h, and the culture continued to incubate for another 4 h at 37 °C. After the medium has been removed, cells and dye crystals are solubilized with 200 μl dimethylsulfoxide (DMSO), and optical density is measured at 570 nm.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Adult male C57BL/6 mice
  • Dosages: 5 and 20 mg/kg
  • Administration: p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (127.1 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 786.73
Formula

C35H46O20

CAS No. 82854-37-3
Storage powder
in solvent
Synonyms N/A
Smiles CC1C(C(C(C(O1)OC2C(C(OC(C2OC(=O)C=CC3=CC(=C(C=C3)O)O)COC4C(C(C(C(O4)CO)O)O)O)OCCC5=CC(=C(C=C5)O)O)O)O)O)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID