Name: Irinotecan hydrochloride
Brand: Selleck Chemicals
SKU: S5026
Rating: 5 (19 reviews)

Irinotecan (CPT-11, (+)-Irinotecan) hydrochloride is an inhibitor of Topoisomerase I (Topo I) that exhibits cytotoxicity in LoVo cells and HT-29 cells with IC50 of 15.8 μM and 5.17 μM, respectively.

Irinotecan hydrochloride Chemical Structure

CAS: 100286-90-6

Selleck's Irinotecan hydrochloride has been cited by 19 publications

Purity & Quality Control

Batch: Purity: 99.99%

99.99

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Signaling Pathway

Topoisomerase Signaling Pathway

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description

Irinotecan (CPT-11, (+)-Irinotecan) hydrochloride is an inhibitor of Topoisomerase I (Topo I) that exhibits cytotoxicity in LoVo cells and HT-29 cells with IC50 of 15.8 μM and 5.17 μM, respectively.

Targets

Topo I ^[1]	cytotoxicity in HT-29 cells ^[1] (Cell-based assay)	cytotoxicity in LoVo cells ^[1] (Cell-based assay)
	5.17 μM	15.8 μM

In vitro
In vitro	The combination of gefitinib and irinotecan has a significant synergistic effect on the proliferation and migration of MDA-MB-231 cells.^[2]
Cell Research	Cell lines	Human BC cell lines
	Concentrations	32 μM
	Incubation Time	24 h, 72 h
	Method	Cells are seeded in 96-well plates at 6 × 103 cells/well for overnight incubation. The cells are exposed to irinotecan alone or combinations. Seventy-two hours after drug treatment, cell viability is tested with the MTT assay. For wound fealing assay, cells are seeded in 6-well culture plates at a density of 3 × 10⁵ cells/mL. After growing to confluence, cells are treated with irinotecan for 48 h in DMEM.

In Vivo
In vivo	The combination of gefitinib and irinotecan has a significant synergistic effect in the treatment of TNBC subtype cells in xenograft model.^[2]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05854498	Recruiting	Metastatic Colorectal Cancer	University of Wisconsin Madison\|Ipsen	October 13 2023	Phase 2
NCT05732129	Not yet recruiting	Homologous Recombination Deficiency Alterations Metastatic Colorectal Cancer	Fudan University	March 1 2023	Phase 2
NCT05731518	Recruiting	Small Cell Lung Cancer	Biocity Biopharmaceutics Co. Ltd.	February 23 2023	Phase 1\|Phase 2
NCT06003998	Recruiting	Colorectal Cancer\|Peritoneal Metastases	Catharina Ziekenhuis Eindhoven	December 27 2022	Phase 2
NCT05277766	Recruiting	Peritoneal Carcinomatosis\|Peritoneal Metastases\|Colorectal Cancer\|Small Bowel Cancer\|Appendix Cancer\|Gastric Cancer\|Pancreatic Cancer\|Bile Duct Cancer	University Hospital Ghent\|Kom Op Tegen Kanker\|University Ghent	November 21 2022	Phase 1

References

Chemical Information & Solubility

Molecular Weight	623.14	Formula	C₃₃H₃₉ClN₄O₆
CAS No.	100286-90-6	SDF	--
Smiles	Cl.CCC1=C2CN3C(=O)C4=C(C=C3C2=NC5=C1C=C(OC(=O)N6CCC(CC6)N7CCCCC7)C=C5)C(O)(CC)C(=O)OC4
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (160.47 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5%DMSO 1 Corn oil	5.0mg/ml (8.02mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml (8.02mM)
Clear solution	2% DMSO 1 40% PEG 300 2 5%Tween80 3 53%ddH2O	2.0mg/ml (3.21mM)	Taking the 1 mL working solution as an example, add 20 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 530 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	2%DMSO 1 98%Corn oil	0.24mg/ml (0.39mM)	Taking the 1 mL working solution as an example, add 20 μL of 12 mg/ml clear DMSO stock solution to 980 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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