GC7 Sulfate

Catalog No.S2961 Synonyms: N1-guanyl-1,7-diaminoheptane Sulfate

For research use only.

GC7 (N1-guanyl-1,7-diaminoheptane) Sulfate is an inhibitor of deoxyhypusine synthase (DHPS). GC7 inhibits Neuroblastoma (NB) cell proliferation in a dose-dependent manner, through induction of the cell cycle inhibitor p21 and reduction of total and phosphorylated Rb proteins.

GC7 Sulfate Chemical Structure

CAS No. 150417-90-6

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Biological Activity

Description GC7 (N1-guanyl-1,7-diaminoheptane) Sulfate is an inhibitor of deoxyhypusine synthase (DHPS). GC7 inhibits Neuroblastoma (NB) cell proliferation in a dose-dependent manner, through induction of the cell cycle inhibitor p21 and reduction of total and phosphorylated Rb proteins.
Targets
DHPS [1]
In vitro

GC7 Sulfate, a deoxyhypusine synthase (DHPS) inhibitor, inhibits MYCN-amplified and chemotherapy-resistant NB cell proliferation, regulates several cell cycle controlling proteins and triggers arrest via the p21/Cdk4/Rb signaling axis, even in the presence of MYCN.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: MYCN2 (±Dox) and BE(2)-C cells
  • Concentrations: 0.1-100 μM
  • Incubation Time: 72 h
  • Method:

    Cell viability was analyzed with the MTS assay kit. Briefly, after treatment with GC7 inhibitor for 72 h in 96-well plates, cells were directly incubated with the MTS dye reagent for 1 h at 37 ℃ in a 5 % CO2 atmosphere.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 270.35
Formula

C8H22N4O4S

CAS No. 150417-90-6
Storage 3 years -20°C powder
2 years -80°C in solvent

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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