Furazolidone

Catalog No.S4528

For research use only.

Furazolidone is a synthetic nitrofuran derivative with bactericidal or bacteriostatic activity when used against Gram-positive and Gram-negative bacteria.

Furazolidone Chemical Structure

CAS No. 67-45-8

Selleck's Furazolidone has been cited by 1 Publication

Purity & Quality Control

Choose Selective Bacterial Inhibitors

Biological Activity

Description Furazolidone is a synthetic nitrofuran derivative with bactericidal or bacteriostatic activity when used against Gram-positive and Gram-negative bacteria.
In vitro

Furazolidone displays potent antiproliferative properties at submicromolar concentrations and induces apoptosis in AML cell lines. Furazolidone treatment of certain AML cells induces myeloid cell differentiation by morphology and flow cytometry for CD11b expression, resulting in increased stability of tumor suppressor p53 protein in AML cells[1].

In vivo FZ accelerates its own metabolism in the chicken by induction of the activity of CPR whereas no effect is observed in the rat[2].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: The leukemic cells(Kasumi-1, NB4 and MolM-13)
  • Concentrations: from 1 µM to 50 µM
  • Incubation Time: 24, 48 and 72 hours
  • Method:

    Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×104 viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of FZD ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with FZD for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate.

Animal Research:

[2]

  • Animal Models: Wistar rats and White Leghorn chickens
  • Dosages: 62.5 mg/kg/day; 125 mg/kg/day
  • Administration: orally by crop tube

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 225.16
Formula

C8H7N3O5

CAS No. 67-45-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COC(=O)N1N=CC2=CC=C(O2)[N+](=O)[O-]

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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