Furazolidone

Catalog No.S4528 Batch:S452801

Technical Data

Formula

C₈H₇N₃O₅

Molecular Weight

225.16

CAS No.

67-45-8

Solubility (25°C)*

In vitro

DMSO

45 mg/mL (199.85 mM)

Water

Insoluble

Ethanol

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.75mg/ml (3.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.11mg/ml (0.49mM)	Taking the 1 mL working solution as an example, add 50 μL of 2.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Furazolidone (Furoxone) is a synthetic nitrofuran derivative with bactericidal or bacteriostatic activity when used against Gram-positive and Gram-negative bacteria.This compound can be used to induce animal models of Cardiomyopathy.
In vitro	Furazolidone displays potent antiproliferative properties at submicromolar concentrations and induces apoptosis in AML cell lines. This compound treatment of certain AML cells induces myeloid cell differentiation by morphology and flow cytometry for CD11b expression, resulting in increased stability of tumor suppressor p53 protein in AML cells.
In vivo	Furazolidone accelerates its own metabolism in the chicken by induction of the activity of CPR whereas no effect is observed in the rat.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines The leukemic cells(Kasumi-1, NB4 and MolM-13) Concentrations from 1 µM to 50 µM Incubation Time 24, 48 and 72 hours Method Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×10⁴ viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of Furazolidone ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with this compound for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate.
Animal Study:^{^[2]}	Animal Models Wistar rats and White Leghorn chickens Dosages 62.5 mg/kg/day; 125 mg/kg/day Administration orally by crop tube

Cell Assay:^{^[1]}

Cell lines
The leukemic cells(Kasumi-1, NB4 and MolM-13)
Concentrations
from 1 µM to 50 µM
Incubation Time
24, 48 and 72 hours
Method
Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×10⁴ viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of Furazolidone ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with this compound for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate.

Animal Study:^{^[2]}

Animal Models
Wistar rats and White Leghorn chickens
Dosages
62.5 mg/kg/day; 125 mg/kg/day
Administration
orally by crop tube

References

https://pubmed.ncbi.nlm.nih.gov/23951311/
http://www.sciencedirect.com/science/article/pii/S0048357511000423

Selleck's Furazolidone Has Been Cited by 2 Publications

The anti-infection drug furazolidone inhibits NF-κB signaling and induces cell apoptosis in small cell lung cancer [ Kaohsiung J Med Sci, 2020, 10.1002/kjm2.12281]	PubMed: 32767507
The anti-infection drug furazolidone inhibits NF-κB signaling and induces cell apoptosis in small cell lung cancer [ Kaohsiung Journal of Medical Sciences, 2020, 998-1003]	PubMed: 32767507

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.