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Calcium folinate (Leucovorin calcium) DHFR inhibitor

Name: Calcium folinate (Leucovorin calcium)
Brand: Selleck Chemicals
SKU: S5136
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S5136

Calcium folinate (Leucovorin calcium) is a metabolically active, reduced form of folic acid that is rapidly absorbed and extensively metabolized in the liver to other folic acid derivatives.Solutions are unstable and should be fresh-prepared.

Chemical Structure

Molecular Weight: 511.5

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Quality Control

Batch: Purity: 99.72%

99.72

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other DHFR Inhibitors	Calcium Folinate Aminopterin Diaveridine

Solubility

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	511.5	Formula	C₂₀H₂₁N₇O₇.Ca	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1492-18-8	Download SDF		Storage of Stock Solutions	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

Read more about storage stability CAS number

Mechanism of Action

In vivo	Calcium folinate (also known as Leucovorin) treatment has no effect on tumor growth. The combination of bortezomib and leucovorin is able to induce apoptosis and attenuate tumor growth in vivo.
References	[1] https://pubmed.ncbi.nlm.nih.gov/2260989/ [2] https://www.nature.com/articles/s41598-017-00839-9

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-08-25)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03959085	RECRUITING	B Acute Lymphoblastic Leukemia; B Lymphoblastic Lymphoma; Central Nervous System Leukemia; Mixed Phenotype Acute Leukemia; Testicular Leukemia	Children's Oncology Group	2019-10-31	PHASE3
NCT03801876	ACTIVE_NOT_RECRUITING	Clinical Stage I Esophageal Adenocarcinoma AJCC v8; Clinical Stage I Esophageal Squamous Cell Carcinoma AJCC v8; Clinical Stage I Gastroesophageal Junction Adenocarcinoma AJCC v8; Clinical Stage II Esophageal Adenocarcinoma AJCC v8; Clinical Stage II Esophageal Squamous Cell Carcinoma AJCC v8; Clinical Stage II Gastroesophageal Junction Adenocarcinoma AJCC v8; Clinical Stage III Esophageal Adenocarcinoma AJCC v8; Clinical Stage III Esophageal Squamous Cell Carcinoma AJCC v8; Clinical Stage III Gastroesophageal Junction Adenocarcinoma AJCC v8; Clinical Stage IVA Esophageal Adenocarcinoma AJCC v8; Clinical Stage IVA Esophageal Squamous Cell Carcinoma AJCC v8; Clinical Stage IVA Gastroesophageal Junction Adenocarcinoma AJCC v8; Thoracic Esophagus Squamous Cell Carcinoma	NRG Oncology	2019-06-26	PHASE3
NCT07636564	NOT_YET_RECRUITING	Acute Leukemia of Ambiguous Lineage; B Acute Lymphoblastic Leukemia; B Acute Lymphoblastic Leukemia, Philadelphia Chromosome Negative; T Acute Lymphoblastic Leukemia	SWOG Cancer Research Network	2026-10-14	PHASE2
NCT07271355	NOT_YET_RECRUITING	Metastatic Appendix Carcinoma; Metastatic Colorectal Carcinoma; Metastatic Malignant Neoplasm in the Peritoneum; Stage IV Appendix Carcinoma AJCC v8; Stage IVC Colorectal Cancer AJCC v8; Unresectable Colorectal Carcinoma	City of Hope Medical Center	2026-10-01	PHASE3
NCT00004317	RECRUITING	Toxoplasmosis	National Institute of Allergy and Infectious Diseases (NIAID)	2000-07	PHASE4
NCT06958328	RECRUITING	Locally Advanced Unresectable Pancreatic Ductal Adenocarcinoma; Stage II Pancreatic Cancer AJCC v8; Stage III Pancreatic Cancer AJCC v8; Stage IV Pancreatic Cancer AJCC v8	NRG Oncology	2025-08-21	PHASE3

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