Calcium folinate

For research use only.

Catalog No.S5136 Synonyms: Leucovorin calcium, Folinic acid calcium salt, Wellcovorin

Calcium folinate Chemical Structure

CAS No. 1492-18-8

Calcium folinate (Leucovorin calcium, Folinic acid calcium salt, Wellcovorin) is a metabolically active, reduced form of folic acid that is rapidly absorbed and extensively metabolized in the liver to other folic acid derivatives.

Purity & Quality Control

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Biological Activity

Description Calcium folinate (Leucovorin calcium, Folinic acid calcium salt, Wellcovorin) is a metabolically active, reduced form of folic acid that is rapidly absorbed and extensively metabolized in the liver to other folic acid derivatives.
In vivo Calcium folinate (also known as Leucovorin) treatment has no effect on tumor growth. The combination of bortezomib and leucovorin is able to induce apoptosis and attenuate tumor growth in vivo[2].

Protocol

Cell Research:[1]
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  • Cell lines: MCF-7 cells
  • Concentrations: 0-100 μM
  • Incubation Time: 10 days
  • Method: The breast cells are grown in RPM11640 medium supplemented with 10% dialyzed fetal calf serum and 2 mM glutamine at an initial plating density of 500-1000 cells/plate. After 12 hr, the cells are treated for a 24-hr period with various MTX concentrations: 0.5, 1, 3, 10 μM. At the end of the 24-hr MTX exposure, the cells are washed two times with phosphate-buffered saline and placed in drug-free medium containing various concentrations of leucovorin. Colonies are allowed to grow for a lo-day period prior to staining and counting. Cloning efficiency is determined by comparing the number of colonies formed in MTX-treated cells to those of a control without MTX, but with exposure the corresponding leucovorin concentration.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Human colorectal HCT116 xenograft tumors (female NSG mice)
  • Dosages: 80 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Water 100 mg/mL (195.5 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 511.5
Formula

C20H21N7O7.Ca

CAS No. 1492-18-8
Storage powder
in solvent
Synonyms Leucovorin calcium, Folinic acid calcium salt, Wellcovorin
Smiles C1C(N(C2=C(N1)N=C(NC2=O)N)C=O)CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)[O-])C(=O)[O-].[Ca+2]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04060030 Recruiting Drug: Levoleucovorin Calcium|Other: Placebo Autism Spectrum Disorder|Language Disorders Phoenix Children''s Hospital|Autism Speaks|State University of New York - Downstate Medical Center|University of Arizona|New York State Institute for Basic Research October 8 2020 Phase 2
NCT04060017 Recruiting Drug: Levoleucovorin Calcium|Other: Placebo Autism Spectrum Disorder|Language Disorders Phoenix Children''s Hospital|State University of New York - Downstate Medical Center|United States Department of Defense|University of Arizona|New York State Institute for Basic Research September 22 2020 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID