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Baohuoside I

Name: Baohuoside I
Brand: Selleck Chemicals
SKU: S3840
Rating: 5 (1 reviews)

Synonyms: Icariside II

Catalog No.S3840 Purity: 99.96%

Baohuoside I (Icariside II) is a flavonoid isolated from Epimedium koreanum Nakai with anti-inflammatory and anti-cancer activities. It may exert cytotoxic effect via the ROS/MAPK pathway.

Baohuoside I Chemical Structure

CAS: 113558-15-9

Purity & Quality Control

Batch: S384001 DMSO] 100 mg/mL] false] ] ] false] ] ] false Purity: 99.96%

99.96

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Biological Activity

Description	Baohuoside I (Icariside II) is a flavonoid isolated from Epimedium koreanum Nakai with anti-inflammatory and anti-cancer activities. It may exert cytotoxic effect via the ROS/MAPK pathway.

In vitro
In vitro	Baohuoside I accelerates apoptosis through the mitochondrial apoptotic pathway, involving the increment of BAX/Bcl-2 ratio, dissipation of mitochondrial membrane potential, transposition of cytochrome c, caspase 3 and caspase 9 activation, degradation of poly (ADP-ribose) polymerase and the over-production of reactive oxygen species (ROS)^[1]. In vitro studies with three leukaemia and three solid tumour cell lines show that Baohuoside I has cytotoxic properties, with IC50 values of between 2.8 and 7.5 μg/ml^[2].
Cell Research	Cell lines	A549 cell
	Concentrations	6.25, 12.5, and 25 μM
	Incubation Time	24 h
	Method	Cells (1×10⁶ cells/ml) are exposed to Baohuoside I (6.25, 12.5, and 25 μM) for 24 h, washed with PBS, and then harvested with trypsin EDTA, counted, fixed with 75% ethanol and stored at 4℃ prior to DNA content analyses. Samples are resuspended in 1.0 ml hypotonic propidium iodide (PI)-solution (50 mg/ml PI, 0.1% sodium citrate, 0.1% Triton X-100, 0.1 mg/ml ribonuclease A) and incubated in the dark for 1 h. PI-stained samples are analyzed with a flow cytometer.

In Vivo
In vivo	Baohuoside I is both anti-inflammatory and immunosuppressive, but unlike the cytotoxic and anti-rejection drugs it suppresses only B-cell (antibody) and not T-cell responses. Baohuoside I may have a role in therapy of those autoimmune diseases^[2].
Animal Research	Animal Models	Inbred female C57/BL/6J mice
	Dosages	20, 40, 60 mg/kg
	Administration	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT02931305	Unknown status	Osteoporosis\|Cardiovascular Disease	National University Hospital Singapore	October 2016	Phase 1

References

Chemical Information & Solubility

Molecular Weight	514.52	Formula	C₂₇H₃₀O₁₀
CAS No.	113558-15-9	SDF	Download Baohuoside I SDF
Smiles	CC1C(C(C(C(O1)OC2=C(OC3=C(C(=CC(=C3C2=O)O)O)CC=C(C)C)C4=CC=C(C=C4)OC)O)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (194.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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