alpha-Asarone

Catalog No.S4772 Synonyms: trans-Asarone

For research use only.

Alpha (α)-asarone (trans-Asarone) is one of the main pharmacologically active compounds present in Acorus calamus Linn (Acoraceae), Acorus tatarinowii Schott (Acoraceae), and Acorus gramineus Solander. It has anticonvulsant, neuroprotective, anxiolytic and nootropic effects.

alpha-Asarone Chemical Structure

CAS No. 2883-98-9

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Biological Activity

Description Alpha (α)-asarone (trans-Asarone) is one of the main pharmacologically active compounds present in Acorus calamus Linn (Acoraceae), Acorus tatarinowii Schott (Acoraceae), and Acorus gramineus Solander. It has anticonvulsant, neuroprotective, anxiolytic and nootropic effects.
In vitro

α-asarone possesses a endothelium-dependent vasodilation property, and in normal HUVEC-derived EA.hy926 cells, α-asarone has been shown to upregulate the expression of phosphorylated eNOS (Ser1177), causing an increase in the production of NO. It protects against injury to endothelial cells by attenuating Ang II-mediated changes in intracellular ROS/NO[1].

In vivo Alpha-Asarone treatment alleviates locomotor hyperactivity in Fmr1 KO mice. It improves m1 mAChR expression and up-regulated the ACh levels in the striatum. Its treatment also attenuates the increased striatal AChE activity in Fmr1 KO mice. Alpha-asarone has been reported to have a beneficial effectin cognitive impairment induced by dysfunction of the cholinergicsystem in the brain through inhibition of AChE activity[2]. Of all the doses tested (2, 10, 40, 80 and 120 mg/kg) in rats, 10 mg/kg α-Asarone improves the quality of sleep, as indicated by an increased NREM bout duration, reduced arousal index, and decreased bout frequencies of NREM sleep and wakefulness. A marginal decrease in the hypothalamic and body temperatures is also observed. Higher doses, on the other hand, not only reduce the quantity and quality of sleep, but also produce hypothermia[3].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: EA.hy926 cell line
  • Concentrations: 10, 50, 100 µmol/L
  • Incubation Time: 1 h
  • Method: EA.hy926 cells are subcultured at 50,000/well in 96-well plates as in the experiment above and pretreated with α-asarone at different concentrations (10, 50, and 100 μmol/L) for 1 h. Subsequently, Ang II (0.1 μmol/L) is added to the cultures and incubated for 24 h at 37°C. Thereafter, the cells are loaded with the fluorescent probes for 30 min, and then the extracellular residual dyes are washed away before detection.
Animal Research:[2]
  • Animal Models: Fmr1 knockout (KO) mice (male, 6–8 weeks of age)
  • Dosages: 10 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 208.25
Formula

C12H16O3

CAS No. 2883-98-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC=CC1=CC(=C(C=C1OC)OC)OC

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