Pentylenetetrazol

For research use only.

Catalog No.S4587 Synonyms: Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ

Pentylenetetrazol Chemical Structure

CAS No. 54-95-5

Pentylenetetrazol (Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ) displays activity as a central nervous system and respiratory stimulant. It is considered a non-competitive GABA(A) receptor antagonist.

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Biological Activity

Description Pentylenetetrazol (Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ) displays activity as a central nervous system and respiratory stimulant. It is considered a non-competitive GABA(A) receptor antagonist.
Targets
GABA receptor [1]
In vivo Administration of PTZ (45 mg/kg i.p.) induces clonic convulsions in almost all of the animals after a single injection, typically within 3 min. However, 11% of animals need a second injection to cause clonus. Each rat shows jerking or myoclonus followed by clonus or tonic-clonic convulsions. PTZ injection results in significant changes in GABAA receptor subunit mRNAs. A single PTZ-induced convulsion is associated with rapid changes in GABAA receptors[1].

Protocol

Cell Research:[2]
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  • Cell lines: primary cortical neurons isolated from E16 mice embryos
  • Concentrations: 0, 7.5, 15, 30, and 60 mM
  • Incubation Time: 24 h
  • Method: Primary cortical neurons are obtained from E16 mice embryos and planted in poly-l-lysine and Laminin coated culturing ware in neurobasal medium with 2% B-27 supplement, 0.5% GlutaMAX-ITM supplement, and 1% penicillin-streptomycin. The culture medium is half replaced every 3 days. The primary cells are cultured for 7 days in vitro (DIV) prior to treating them with isometric fresh medium, PTZ (30 mM), and PTZ (30 mM) + OtoN of different dosages.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Male Sprague-Dawley rats
  • Dosages: 45 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 27 mg/mL (195.41 mM)
Water 27 mg/mL (195.41 mM)
Ethanol '27 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 138.17
Formula

C6H10N4

CAS No. 54-95-5
Storage powder
in solvent
Synonyms Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ
Smiles C1CCC2=NN=NN2CC1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID