BVT-948

Catalog No.S9829

For research use only.

BVT 948 is a irreversible, noncompetitive and cell-permeable protein tyrosine phosphatase(PTP) inhibitor with IC50s of 0.9 μM, 1.7 μM, 0.09 μM, 1.5 μM and 0.7 μM for PTP1B, TCPTP, SHP-2, LAR and YopH respectively.

BVT-948 Chemical Structure

CAS No. 39674-97-0

Purity & Quality Control

Choose Selective phosphatase Inhibitors

Biological Activity

Description BVT 948 is a irreversible, noncompetitive and cell-permeable protein tyrosine phosphatase(PTP) inhibitor with IC50s of 0.9 μM, 1.7 μM, 0.09 μM, 1.5 μM and 0.7 μM for PTP1B, TCPTP, SHP-2, LAR and YopH respectively.
Targets
SHP-2 [1]
(Cell-free assay)
YopH [1]
(Cell-free assay)
PTP1B [1]
(Cell-free assay)
LAR [1]
(Cell-free assay)
TCPTP [1]
(Cell-free assay)
0.09 μM 0.7 μM 0.9 μM 1.5 μM 1.7 μM
In vitro

In L6 myotubes, PTP1B is oxidized further to the irreversible sulfinic and sulfonic forms with BVT.948. BVT.948 is able to increase tyrosine phosphorylation of cellular proteins, including the insulin receptor. BVT.948 inhibits several other PTPs tested and also was efficient at inhibiting several cytochrome P450 (P450) isoforms.[1]

In vivo

BVT.948 enhances insulin tolerance tests in ob/ob mice, therefore apparently enhancing insulin sensitivity. BVT.948 can significantly enhance glucose clearance from the blood stream in response to insulin. [1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: L6 myotubes
  • Concentrations: 25 μM
  • Incubation Time: 30 min
  • Method:

    L6 myotubes are starved of serum overnight and stimulated with insulin or treated with 25 M BVT.948 after a 30-min preincubation with (dark gray bars) or without (light gray bars) 100 nM wortmannin. After washing, glucose uptake was measured.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: ob/ob mice
  • Dosages: 0.3 μmol/kg, 3 μmol/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 48 mg/mL
(198.97 mM)
Ethanol 5 mg/mL
(20.72 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 241.24
Formula

C14H11NO3

CAS No. 39674-97-0
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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