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FB23 FTO inhibitor

Name: FB23
Brand: Selleck Chemicals
SKU: S6291
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S6291

FB23 is a potent and selective inhibitor of FTO demethylase with IC50 of 60 nM. This compound directly binds to FTO and selectively inhibits FTO's mRNA N6-methyladenosine (m6A) demethylase activity.

Chemical Structure

Molecular Weight: 377.22

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Quality Control

Batch: Purity: 99.52%

99.52

Related Targets	PRMT EZH2 MAT2A DNA Methyltransferase LSD1 JMJD G9a/GLP MLL NSD SETD
Other FTO Inhibitors	FB23-2 Dac51 Rheic Acid Bisantrene (CS1)

Solubility

In vitro
Batch:

DMSO : 38 mg/mL (100.73 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.900mg/ml (5.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 38 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.900mg/ml (5.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 38 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	377.22	Formula	C₁₈H₁₄Cl₂N₂O₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2243736-35-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC1=C(C(=NO1)C)C2=CC(=C(NC3=CC=CC=C3C(O)=O)C(=C2)Cl)Cl

Mechanism of Action

Targets/IC₅₀/Ki	FTO demethylase (Cell-free assay) 60 nM
In vitro	FB23 treatment inhibits acute myeloid leukemia (AML) cells proliferation with IC₅₀ values of 44.8 μM, 23.6 μM for NB4 and MONOMAC6 AML cells. This compound treatment causes the significant suppression of MYC targets, E2F targets, and G2M checkpoint signal cascades, which may contribute to the inhibitory effects of FTO inhibitors and FTO KD on cell cycle and proliferation. This chemical treatments activates apoptosis and p53 pathways.
In vivo	Pharmacological inhibition of FTO by FB23-2 substantially suppresses leukemia progression and prolongs survival.
References	[1] https://pubmed.ncbi.nlm.nih.gov/30991027/

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