Home Methylation FTO inhibitor Dac51 For research use only.

Dac51

Dac51 is a potent FTO inhibitor with an IC50 of 0.4 μM for inhibiting FTO demethylation.

Dac51 Chemical Structure

Dac51 Chemical Structure

CAS: 2243944-92-3 (free base)

Selleck's Dac51 has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.80%
99.80

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Biological Activity

Description Dac51 is a potent FTO inhibitor with an IC50 of 0.4 μM for inhibiting FTO demethylation.
In vitro
In vitro

Dac51 can serve as a potent FTO inhibitor to dampen the glycolytic capacity of tumor cells by inhibiting FTO-mediated demethylation on transcripts including Jun and Cebpb.[1]
Cell Research Cell lines B16-OVA cells, LLC cell line, LLC-zsGreen-OTIp cells, MC38 cell line
Concentrations 1 μM, 5 μM
Incubation Time 48 h
Method

Cells are cultured in Dulbecco s modified Eagles medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, 10 mM HEPES, and 1% non-essential amino acids at 37 ℃ 5% CO2. Cells are cultured with Dac51 (1 mM, 5mM) for 48 hr.Then cell growth is monitored by Trypan Blue staining. Apoptosis is analyzed through flow cytometry. Cell total RNA is extracted for m6A  dot  blot  assay.
In Vivo
In vivo

Dac51, a FTO inhibitor, delivers strong T cell-mediated antitumor effects and prevents tumor recurrence via increasesing CD8+ T cell infiltration in tumors, in multiple vivo cancer models.[1]
Animal Research Animal Models C57BL/6 mice inoculated with Fto-Kd or B16-OVA tumor cells, Rag2/ mice inoculated with B16-OVA cells
Dosages 2 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 505.27 Formula

C20H17Cl2F3N4O4
CAS No. 2243944-92-3 (free base) SDF --
Smiles CC1=C(C(=N[NH]1)C)C2=CC(=C(NC3=CC=CC=C3C(=O)NO)C(=C2)Cl)Cl.OC(=O)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (197.91 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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