FB23-2

Catalog No.S8837

For research use only.

FB23-2 is a potent and selective FTO inhibitor that directly binds to FTO and selectively inhibits FTO's N6-methyladenosine (m6A) demethylase activity with IC50 of 2.6 μM.

FB23-2 Chemical Structure

CAS No. 2243736-45-8

Purity & Quality Control

Choose Selective FTO Inhibitors

Biological Activity

Description FB23-2 is a potent and selective FTO inhibitor that directly binds to FTO and selectively inhibits FTO's N6-methyladenosine (m6A) demethylase activity with IC50 of 2.6 μM.
Targets
FTO [1]
(Cell-free assay)
2.6 μM
In vitro

FB23-2 directly binds to FTO and selectively inhibits FTO’s m6A demethylase activity. Mimicking FTO depletion, FB23-2 dramatically suppresses proliferation and promotes the differentiation/apoptosis of human acute myeloid leukemia (AML) cell line cells and primary blast AML cells in vitro.[1]

In vivo

FB23-2 significantly inhibits the progression of human AML cell lines and primary cells in xeno-transplanted mice.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Leukemia cells NB4, U937, MV4-11, ML-2; MONOMAC6 (ACC-124) cells; 293T cells; The mouse bone marrow cells FLT3ITD/NPM1, MA9
  • Concentrations: 10 μM, 5 μM
  • Incubation Time: 24 h, 72 h
  • Method:

    NB4 and MONOMAC6 cells are treated with DMSO or FB23-2 at varying concentrations for 72 h, while MA9 and FLT3/NPM1 primary cells isolated from AML mice, five human AML cell (MA9.3ITD, MA9.3RAS, U937, ML2, and MV4-11), and human primary AML cells are treated with DMSO or 5 μM FB23-2 for 72 h for dot blot assay.

Animal Research:

[1]

  • Animal Models: NSGS mice with xeno-transplantation model
  • Dosages: 2 mg/kg
  • Administration: IP

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 392.24
Formula

C18H15Cl2N3O3

CAS No. 2243736-45-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=NO1)C)C2=CC(=C(C(=C2)Cl)NC3=CC=CC=C3C(=O)NO)Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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