Fludarabine (NSC 118218)

Catalog No.S1491 Synonyms: FaraA, Fludarabinum

For research use only.

Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.

Fludarabine (NSC 118218) Chemical Structure

CAS No. 21679-14-1

Selleck's Fludarabine (NSC 118218) has been cited by 131 publications

Purity & Quality Control

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Biological Activity

Description Fludarabine (NSC 118218, FaraA, Fludarabinum) is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells. Fludarabine induces apoptosis.
Targets
STAT1 [4]
(Vascular smooth muscle cells)
In vitro

Fludarabine efficiently inhibits the proliferation of RPMI 8226 cells with IC50 of 1.54 μg/mL. The IC50 of Fludarabine against MM.1S and MM.1R cells is 13.48 μg/mL and 33.79 μg/mL, respectively. In contrast, U266 cells are resistant to Fludarabine with IC50 of 222.2 μg/mL. Fludarabine treatment results in increased number of cells in the G1 phase of cell cycle, accompanied with a concomitant reduction of cells at the S phase of cell cycle in a time-dependent manner. Fludarabine induces a cell cycle block and triggers apoptosis in MM cells. Fludarabine triggers time-dependent cleavage of caspase-8, -9, and -3, -7, followed by PARP cleavage. Fludarabine increases expression of Bax in a time-dependent fashion, while the expression of Bak doesn't change. After exposure to Fludarabine for 12 hours, RPMI 8226 cells shows a loss of membrane potential with 61.05% of the cells expressing low fluorescence of rhodamine 123 compared with 8.62% of cells in untreated control. [1] To enhance solubility, Fludarabine is formulated as the monophosphate (F-ara-AMP, fudarabine), which is instantaneously and quantitatively dephosphorylated to the parent nucleoside upon intravenous infusion. Inside the cells rephosphorylation occurs which leads to fuoroadenine arabinoside triphosphate (F-ara-ATP), the major cytotoxic metabolite of F-ara-A. [2] Fludarabine can also induce pro-inflammatory stimulation of monocytic cells, as evaluated by increased expression of ICAM-1 and IL-8 release. [3] Fludarabine does not affect the growth of ovarian cancer cell lines, whereas it induces marked and dose-dependent inhibition of proliferation in melanoma cell lines. [4] Fludarabine induces significant reduction of STAT-1 phosphorylation, whereas it does not change JAK2 activation. Interestingly, Fludarabine does not significantly affect the phosphorylation of these three STAT proteins. Fludarabine (1.5 mg) significantly prevents STAT-1 phosphorylation and also reduces the increased amount of this protein. No significant changes are demonstrated in JAK2 phosphorylation at 2 days, but Fludarabine inhibits JAK2-increased expression at 7 days. Fludarabine specifically inhibits STAT-1 activation without affecting other STAT proteins and consequently diminishes VSMC proliferation. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  MmXGSpVv[3Srb36gRZN{[Xl? NHznU40zOCEQvF2= M3;EOVI1KGh? MWrpcohq[mm2czDlfJBz\XO|aX;uJI9nKEmGTx?= M{TYfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUSwO|EzLz5{NUm0NFcyOjxxYU6=
MV-4-11 Ml3rRZBweHSxc3nzJGF{e2G7 NULhVVNSOi53IN88US=> M2fTOlQ5KGh? M1fEOYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NWrpR3NlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
THP-1 M2Wz[mFxd3C2b4Ppd{BCe3OjeR?= MWGyMlUh|ryP MYG0PEBp Ml;ZbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= NXGwUlV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
MOLM 13 MVjBdI9xfG:|aYOgRZN{[Xl? M1;IVlIvPSEQvF2= M2j6OFQ5KGh? MX3pcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= M3nORVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
KBM3/Bu2506 NV;jSYp5SXCxcITvd4l{KEG|c3H5 MXSyMlUh|ryP MYW0PEBp MljJbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= M4TWe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
Nalm-6 NIHxRlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzlT4FTUUN3ME2xPEDPxE1? MlW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Reh MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37CbGlEPTB;M{Cg{txO Mni1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
U2937 NEfXU5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rjeGlEPTB;MU[g{txO M1zLN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Mec-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3ETWM2OO,:nkWwNEDPxE1? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
RPMI-8226 NGPXOnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvNTWM2OD13MECg{txO NVG3fIVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Molt-4 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrOSZpVUUN3ME2xPFAh|ryP NX:0U3A6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Nalm-6-FluR M3;WNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTJ3MDFOwG0> NEHvcm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Raji  MnnlSpVv[3Srb36gRZN{[Xl? M2LuNFPDqM7:TR?= MnPoNlQwPDhxN{KgbC=> NIK5dmJqdmS3Y3XzJIFk[3WvdXzheIlwdnNib3[gdFU{NCCyNkOgZY5lKHB5M9Mg NWLTdYNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFA3QTVpPkK0PVQxPjl3PD;hQi=>
PBMC MlzsSpVv[3Srb36gRZN{[Xl? M{fxblUxNzFyMDFOwG0> NXyxXm51OjRiaB?= MVPEUXNQ NIf4XWhqdmirYnn0d{BUXEGWMTDwbI9{eGixconsZZRqd25? NXzLfHBsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 NYH0WJo3TnWwY4Tpc44hSXO|YYm= NGHmVFAyODBizszN M4PiclI1KGh? MVnEUXNQ MVnk[YNz\WG|ZYOgTWRQKGW6cILld5Nqd25? M1PJdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUGxPFczLz5{NEmxNVg4OjxxYU6=
624.38mel  MV3GeY5kfGmxbjDBd5NigQ>? NIPsfGU2OCEQvF2= NFLXW|MzPCCq NGTEOlNFVVOR MX3k[YNz\WG|ZYOgTWRQKGW6cILld5Nqd25? M2DkcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUGxPFczLz5{NEmxNVg4OjxxYU6=
MDA-231 NETsR5pHfW6ldHnvckBCe3OjeR?= NYnJNmh5PTBvMkCwJO69VQ>? NGHjN2gzPCCq MX\EUXNQ MUPpcohq[mm2czDJSG8h[WO2aY\peJkhcW6mZYDlcoRmdnSueTDv[kBuWk6DIHzleoVtew>? NF:2ZW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  M3rzUGZ2dmO2aX;uJGF{e2G7 MWS1NE0zODBizszN NV71O|Q3OjRiaB?= NWTIbWJiTE2VTx?= M4[wUolvcGmkaYTzJGlFVyCjY4Tpeol1gSCrbnTldIVv\GWwdHz5JI9nKG2UTlGgcIV3\Wy| NUHic3FRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
HMECs NGrhWVhHfW6ldHnvckBCe3OjeR?= NY\vTXptOTBywrFOwG3DqA>? NEX2dpg{PsLiaB?= NVPVdXRJcW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? NFPTdIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVMzPyd-MkSyNVE{Ojd:L3G+
HMECs  MVvGeY5kfGmxbjDBd5NigQ>? MV2xNFDDqM7:TdMg NXjtVG9pOzcEoHi= MlrRbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUOyO{c,OjR{MUGzNlc9N2F-
BJAB NWX0SmhCSXCxcITvd4l{KEG|c3H5 NGi0dm02yqEQvF2= MnvNNlQhcA>? Ml\XbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NI\ZbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC1O|E1Pyd-MkSwOVcyPDd:L3G+
I-83 NVi3b2tkSXCxcITvd4l{KEG|c3H5 NEPYT3k2yqEQvF2= MnjCNlQhcA>? NGfFZ5lqdmS3Y3XzJINmdGxiYYDvdJRwe2m| NFPyepU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC1O|E1Pyd-MkSwOVcyPDd:L3G+
NALM6 MmfiRZBweHSxc3nzJGF{e2G7 NFmwflQ2yqEQvF2= NIfSXG0zPCCq M{i0Oolv\HWlZYOgZ4VtdCCjcH;weI9{cXN? M1nlTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
DU-145 NV3jbXF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX[wMVExKM7:Zz;tcC=> NFe0d3A1QCCqwrC= NF;tbVlqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M3uzN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{O0PFE2Lz5{M{ezOFgyPTxxYU6=
Nalm-6 NFjGW4xHfW6ldHnvckBCe3OjeR?= MYSxNOKh|ryP M4nPT|EwOi92IHi= NWn2To51cW6mdXPld{BifXSxcHjh[5k> NX7GfpY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Reh M3fzdmZ2dmO2aX;uJGF{e2G7 M2Trb|ExyqEQvF2= MVuxM|IwPCCq NVexXVZRcW6mdXPld{BifXSxcHjh[5k> NYjXWGsyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Nalm-6 MlGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfNbVNKSzVyIPMIwFEx6oDLzszN MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh M{H5W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDjVGFKSzVyIPMIwFEx6oDLzszN MkjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
HEC1A NUjLbm9NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXexNFAuPTByIN88US=> MlP2NlQhcA>? NX3EbnpMcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3jFTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
AN3CA MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETqNmIyODBvNUCwJO69VQ>? MV[yOEBp NWTwSo1wcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NWXYcmMyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B Ml\XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU[zXFNYOTByLUWwNEDPxE1? NUXQbGZbOjRiaB?= M3LoPYlvcGmkaYTzJINmdGxiZ4Lve5RpKHOuaXfoeIx6 M4HuWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
HEC1A MnrnRZBweHSxc3nzJGF{e2G7 MoHMNlAwOTByIN88US=> M3rlZlI1KGh? MUjpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MmXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUW2PVcoRjJ|NUm1Olk4RC:jPh?=
AN3CA Ml\JRZBweHSxc3nzJGF{e2G7 NIPlT|gzOC9zMECg{txO MnW0NlQhcA>? NF\CPHdqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NVPRSYF4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B MYTBdI9xfG:|aYOgRZN{[Xl? NFzud3kzOC9zMECg{txO MW[yOEBp NETtS49qdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
EHEB NYXr[mM4SXCxcITvd4l{KEG|c3H5 MlG0OFAh|ryP NUjIc2pROjRiaB?= NHP6ellqdmS3Y3XzJIFxd3C2b4Ppdy=> M3e0TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3NFc2Lz5{M{S5O|A4PTxxYU6=
A549 NVn4TpdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LXbmlEPTB;MUWuO:KyOi56INM1US=> NV:1[mdTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzO|cyQTJpPkKzN|c4OTl{PD;hQi=>
A549 GAPDH-deficient Mlf2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTF6LkZCtVIvOyEEtV2= NIftRXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O3O|E6Oid-MkOzO|cyQTJ:L3G+
CLL  NYjrO2RFSXCxcITvd4l{KEG|c3H5 MoG5NVAh|ryPwrC= NEDvSngzPC17NjDo MVvpcoR2[2W|IHHwc5B1d3SrYzDj[YxtKGSnYYTo MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJyN{[4Okc,OjJ{MEe2PFY9N2F-
MEC1 Ml20RZBweHSxc3nzJGF{e2G7 NHvjW40yODEEoN88US=> NUf6RmVrPzJiaB?= MWDpcoR2[2W|IHHwc5B1d3OrczDzbYdvcW[rY3HueIx6 MoLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzM{K5O|MoRjJ{MUOyPVc{RC:jPh?=
U937  NUfp[VBOSXCxcITvd4l{KEG|c3H5 NF\5S2UxNjhizszN MWi0MVQ5KGh? MXXpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NFywVm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3OFcxOCd-MkKwO|Q4ODB:L3G+
U937  NGLHV4VCeG:ydH;zbZMhSXO|YYm= NYDIWFJXOSEQvF2= NWLERlE1QTZiaB?= NF3sVo1qdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> M2PHWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEKzOVI{Lz5{MkCyN|UzOzxxYU6=
Daudi MkfURZBweHSxc3nzJGF{e2G7 NHm1b2EzOCEQvF2= M4jRcFk3KGh? M13MWIlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
J45.01 NIrVUZlCeG:ydH;zbZMhSXO|YYm= NX\WfWZ6OSEQvF2= M2DTR|k3KGh? Ml3xbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MmjSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMkO1NlMoRjJ{MEKzOVI{RC:jPh?=
RPMI 8226 M{nNS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jUOWlEPTB;MkWuPeKhyrIEoEOuO{DPxE1? NUDBWJF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OFgzPjRpPkKxPVQ5OjZ2PD;hQi=>
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primary CLL M{HYWmN6fG:2b4jpZ4l1gSCjc4PhfS=> NYXZUmNZS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKrbXHyfUBEVExiY3XscJMtKEyGNUCgQUAyNjFizszNMi=> M4XrS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUS4N|kzLz5{NUG0PFM6OjxxYU6=
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SK-N-MC M1TwS5FJXFNiYYPzZZk> MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= M3\BSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 MW\xTHRUKGG|c3H5 MorGdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NWjnd4lGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 NUnie2l[eUiWUzDhd5NigQ>? NHjBcIdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M1fQZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 Mm\KdWhVWyCjc4PhfS=> MknydWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? MlHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 Mm[4dWhVWyCjc4PhfS=> NEK1T25yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBPSjF4NEOgZ4VtdHN? M4KxeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC M1TqXJFJXFNiYYPzZZk> NGDYVXhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MXzxTHRUKGG|c3H5 NXXpcFh2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NWHvTFRFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 MX;xTHRUKGG|c3H5 NGDoW3hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ NWTvem9xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 NXL6VYxFeUiWUzDhd5NigQ>? MoHDdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVogyQCClZXzsdy=> NHfsS5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 NU\RfYRVeUiWUzDhd5NigQ>? NEDBU3FyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? NEDKZ449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 M17wepFJXFNiYYPzZZk> NVj2VYtteUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz MoS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD MUHxTHRUKGG|c3H5 MnvvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohV3K2aH;nc45idCB|RDD2bYFjcWyrdImgd4Nz\WWwIH\vdkBTTCClZXzsdy=> MmDLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Assay
Methods Test Index PMID
Western blot procaspase-9 / procaspase-3 ; p-p53 / p53 ; STAT1 27223263 26677135
Immunofluorescence α-SMA / Vimentin 28322315
Growth inhibition assay Cell viability 24956101
In vivo Tumors treated with PBS grow rapidly to approx-imately 10-fold their initial volume in 25 day, whereas, the tumors in the Fludarabine at 40 mg/kg increase less than 5-fold. A significant antitumor effect of 40 mg/kg Fludarabine on RPMI8226 tumor growth is demonstrated. RPMI8226 tumors treated with 40 mg/kg Fludarabine at day 10 increase apoptotic nuclei. Fludarabine is effective in suppressing RPMI8226 myeloma xenografts in SCID mice. [1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines
  • Concentrations: 2 μg/mL
  • Incubation Time: 24 hours
  • Method:

    After treated with Fludarabine or control, dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines (5 × 105 cells) are washed twice in phosphate-buffered saline (PBS) and fixed with 70% ice-cold ethanol, then centrifuged and suspended in PBS containing 100 μg/mL RNase A. After incubated for 30 minutes at 37 ºC, samples are resuspended in 25 μg/mL propidium iodide. Flow cytometry is performed on a FACSCalibur automated system. Apoptosis is determined by Annexin V-FITC apoptosis detection kit, according to the manufacturer's instructions. For TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay, cells are analyzed by flow cytometry using the in situ cell death detection kit.

Animal Research:

[1]

  • Animal Models: Severe combined immunodeficient (SCID) mice bearing RPMI 8226 cells
  • Dosages: 40 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.

8 mg/mL

Chemical Information

Molecular Weight 285.23
Formula

C10H12FN5O4

CAS No. 21679-14-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=NC2=C(N=C(N=C2N1C3C(C(C(O3)CO)O)O)F)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2022 Phase 1
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
how to re-suspend and deliver the inhibitor for in vivo experiments?

Answer:
For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

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