6-Aminonicotinamide

Catalog No.S9783 Synonyms: 6AN

For research use only.

6-Aminonicotinamide (6AN) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.

6-Aminonicotinamide Chemical Structure

CAS No. 329-89-5

Selleck's 6-Aminonicotinamide has been cited by 1 Publication

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Biological Activity

Description 6-Aminonicotinamide (6AN) is an antimetabolite used to inhibit the NADPH-producing pentose phosphate pathway (PPP) in many cellular systems, making them more susceptible to oxidative stress. 6-Aminonicotinamide is a competitive inhibitor of NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) with Ki of 0.46 μM.
Targets
G6PD [1]
(Cell-free assay)
0.46 μM(Ki)
In vitro

Blocking the pentose phosphate pathway (PPP) by 6-AN significantly inhibits mES colony formation in the presence of a normal concentration of glucose. 6-AN treatment effectively inhibits AKT phosphorylation and activation of its downstream effector S6. 6-AN treatment has no effect on the ERK and PDK1 activities, suggesting an AKT-specific effect. 6-AN treatment inhibits cell growth.[2]

In vivo

PTEN null human cancer cells and in vivo murine models are sensitive to 6-AN (anti-PPP, anti pentose phosphate pathway) treatments, suggesting the importance of the PPP in maintaining AKT activation even in the presence of a constitutively activated PI3K pathway. The treatment of TALL models with 6-AN significantly increases the Phlda3 mRNA and protein levels, accompanied by substantial decreases in the levels of P-AKT and P-S6 as well as the PPP metabolic intermediates.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: HeLa cells, mES cells
  • Concentrations: 100 nM
  • Incubation Time: 24 h, 2 days, 3 days, 4 days
  • Method:

    PHLDA3 knockout blocks the effects of 6-AN, R5P or uridine on cell growth in vitro. Isogenic PHLDA3 WT and knockout HeLa cells are seeded in 96-well plates at a density of 1000 cells/well. After 48 h, the cells are treated with 6-AN (100 nM). Cell viability is detected by CCK-8 assays at indicated time points.

Animal Research:

[2]

  • Animal Models: male CAnN.Cg-Foxn1nu/CrlVr mice
  • Dosages: 1 mg/kg, 15 mg/kg
  • Administration: IP

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 137.14
Formula

C6H7N3O

CAS No. 329-89-5
Storage 3 years -20°C powder
2 years -80°C in solvent

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