2-Methoxy-1,4-naphthoquinone

Catalog No.S3661

For research use only.

2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.

2-Methoxy-1,4-naphthoquinone Chemical Structure

CAS No. 2348-82-5

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Biological Activity

Description 2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.
Targets
PKCβ1 [1]
()
PKCδ [1]
()
PKCζ [1]
()
In vitro

2-Methoxy-1,4-naphthoquinone (MNQ) exhibits antifungal, antimicrobial activities and showed strong killing effect on K562, HL-60, Raji, MCF-7, H-23, IMR-32, LA-174-T, HSC-2, and SK-MEL-28. MNQ is able to trigger the intrinsic apoptotic pathway in K562 cells, as well as causing p53-dependent cell cycle arrest in the p53-wild type cell line. MNQ inhibits the TCF/β-catenin (TOP) transcriptional activity at IC50 2.9 μM, while it decreases the transcriptional activity of FOP (mutated TCF-binding site)-transfected cell. MNQ exerts an anti-metastatic effect against MDA-MB-231 cells. MNQ has remarkable selectivity towards PKCβI and ζ suppression. MNQ suppresses PKCδ moderately, where MNQ suppresses half of the PKCδ expression at the concentration of 10 μM. MNQ does not suppress PKC βII[1]. MNQ is able to inhibit the invasion and migration characteristics of a highly metastatic MDA-MB-231 cancer cell line[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Raji cells
  • Concentrations: --
  • Incubation Time: 48 h
  • Method: 5×105 Raji cells are incubated in 1 mL of RPMI 1640 medium (supplemented with 10% FBS) containing 0.05 μM PMA, 3 mM SnB and test substance (MNQ or DMSO as the vehicle control) at 37 °C under 5% CO2 for 48 hours. Genistein is used as the standard drug. The cells are then harvested, washed with PBS and fixed onto Teflon-coated slides.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 37 mg/mL
(196.62 mM)
Ethanol 3 mg/mL
(15.94 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 188.18
Formula

C11H8O3

CAS No. 2348-82-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC(=O)C2=CC=CC=C2C1=O

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