Home Ubiquitin E3 Ligase inhibitor - Apoptosis related activator SZL P1-41 For research use only.

SZL P1-41

SZL P1-41 (compound #25) is a specific inhibitor of S-phase kinase-associated protein 2 (Skp2) that indeed binds to Skp2, prevents Skp2-Skp1 interaction and inhibits Skp2 SCF E3 ligase activity, which consequently suppresses survival of cancer cells and cancer stem cells. SZL P1-41 causes higher apoptosis rates in cancer cells.

SZL P1-41 Chemical Structure

SZL P1-41 Chemical Structure

CAS: 222716-34-9

Selleck's SZL P1-41 has been cited by 1 publication

Purity & Quality Control

Batch: S972401 DMSO] 5 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.57%
99.57

SZL P1-41 Related Products

Signaling Pathway

E3 Ligase Signaling Pathways

E3 Ligase Signaling Pathway

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Biological Activity

Description SZL P1-41 (compound #25) is a specific inhibitor of S-phase kinase-associated protein 2 (Skp2) that indeed binds to Skp2, prevents Skp2-Skp1 interaction and inhibits Skp2 SCF E3 ligase activity, which consequently suppresses survival of cancer cells and cancer stem cells. SZL P1-41 causes higher apoptosis rates in cancer cells.
Targets
Skp2 [1]
In vitro
In vitro

SZL P1-41 indeed binds to Skp2, prevents Skp2-Skp1 interaction and inhibits Skp2 SCF E3 ligase activity, which consequently suppresses survival of cancer cells and cancer stem cells.[1]
Cell Research Cell lines PC3 cells
Concentrations 10 μM, 20 μM
Incubation Time 4 days
Method

For Skp2 inhibitor administration, 5~10 × 103 of cells are seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 (refreshed every two days). After four days, cells are harvested, stained with 0.4% trypan blue solution, and viable cells are counted using hemocytometer under microscope by trypan-blue dye exclusion method. For doxorubicin treatment, 8× 103 of PC3 cells with were seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 in the presence or absence of 10nM doxorubicin or 25 ng/ml cyclophosphamide for four and three days, respectively (refreshed every two days).
In Vivo
In vivo

SZL P1-41 also exhibits potent anti-tumor activity in animal studies.[1]
Animal Research Animal Models nude mice bearing A549 xenograft tumors, nude mice bearing PC3 xenograft tumors
Dosages 80 mg/kg, 40 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 420.52 Formula

C24H24N2O3S
CAS No. 222716-34-9 SDF --
Smiles CCC1=C(O)C(=C2OC=C(C(=O)C2=C1)C3=NC4=C(S3)C=CC=C4)CN5CCCCC5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 5 mg/mL ( (11.89 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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