SZL P1-41

Catalog No.S9724 Batch:S972401

Technical Data

Formula	C₂₄H₂₄N₂O₃S
Molecular Weight	420.52	CAS No.	222716-34-9
Solubility (25°C)*	In vitro	DMSO	5 mg/mL (11.89 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

SZL P1-41 (compound #25) is a specific inhibitor of S-phase kinase-associated protein 2 (Skp2) that indeed binds to Skp2, prevents Skp2-Skp1 interaction and inhibits Skp2 SCF E3 ligase activity, which consequently suppresses survival of cancer cells and cancer stem cells. SZL P1-41 causes higher apoptosis rates in cancer cells.

Targets

Skp2 ^[1]

In vitro

SZL P1-41 indeed binds to Skp2, prevents Skp2-Skp1 interaction and inhibits Skp2 SCF E3 ligase activity, which consequently suppresses survival of cancer cells and cancer stem cells.^[1]

In vivo

SZL P1-41 also exhibits potent anti-tumor activity in animal studies.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PC3 cells Concentrations 10 μM, 20 μM Incubation Time 4 days Method For Skp2 inhibitor administration, 5~10 × 10³ of cells are seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 (refreshed every two days). After four days, cells are harvested, stained with 0.4% trypan blue solution, and viable cells are counted using hemocytometer under microscope by trypan-blue dye exclusion method. For doxorubicin treatment, 8× 10³ of PC3 cells with were seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 in the presence or absence of 10nM doxorubicin or 25 ng/ml cyclophosphamide for four and three days, respectively (refreshed every two days).
Animal Study:^{^[1]}	Animal Models nude mice bearing A549 xenograft tumors, nude mice bearing PC3 xenograft tumors Dosages 80 mg/kg, 40 mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
PC3 cells
Concentrations
10 μM, 20 μM
Incubation Time
4 days
Method
For Skp2 inhibitor administration, 5~10 × 10³ of cells are seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 (refreshed every two days). After four days, cells are harvested, stained with 0.4% trypan blue solution, and viable cells are counted using hemocytometer under microscope by trypan-blue dye exclusion method. For doxorubicin treatment, 8× 10³ of PC3 cells with were seeded in 12 wells in triplicate for 24 h and then treated with various doses of SZL P1-41 in the presence or absence of 10nM doxorubicin or 25 ng/ml cyclophosphamide for four and three days, respectively (refreshed every two days).

Animal Study:^{^[1]}

Animal Models
nude mice bearing A549 xenograft tumors, nude mice bearing PC3 xenograft tumors
Dosages
80 mg/kg, 40 mg/kg
Administration
IP

References

[1] Chia-Hsin Chan, et al. Cell. 2013 Aug 1;154(3):556-68.

Selleck's SZL P1-41 has been cited by 2 publications

Gastrodin overcomes chemoresistance via inhibiting Skp2-mediated glycolysis [ Cell Death Discov, 2023, 9(1):364]	PubMed: 37779163
Gastrodin overcomes chemoresistance via inhibiting Skp2-mediated glycolysis [ Cell Death Discov, 2023, 9(1):364]	PubMed: 37779163

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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