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Methyl gallate

Name: Methyl gallate
Brand: Selleck Chemicals
SKU: S3790
Rating: 5 (1 reviews)

Synonyms: Methylgallate, Gallic acid methyl ester, Gallicin

Catalog No.S3790 Purity: 99.98%

Methyl Gallate (Methylgallate, Gallic acid methyl ester, Gallicin) is a plant polyphenol with antioxidant, anticancer, and anti-inflammatory activities.

Methyl gallate Chemical Structure

CAS: 99-24-1

Purity & Quality Control

Batch: Purity: 99.98%

99.98

Methyl gallate Related Products

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Biological Activity

Description	Methyl Gallate (Methylgallate, Gallic acid methyl ester, Gallicin) is a plant polyphenol with antioxidant, anticancer, and anti-inflammatory activities.

In vitro
In vitro	Methyl gallate inhibits Treg cell-suppressive effects on effector CD4+ T cells and Treg migration toward tumor environment. The expression of Treg surface markers including CTLA-4, CCR4, CXCR4, and glucocorticoid-induced TNFR are significantly suppressed upon methyl gallate treatment^[2].
Cell Research	Cell lines	HUVEC
	Concentrations	0-0.5 mM
	Incubation Time	1 h
	Method	HUVECs are grown in 96-well micro plates in a final volume of 100 μl culture medium containing 10⁴ cells per well. After allowing the cultures to grow to confluence, chemical and H2O2 treatments are followed with certain intervals. To determine the cell viability, XTT assay kit is used. Two cases of oxidative stress are applied to pre-incubated HUVECs with chemical for 1 h: the treatment with 1 mM H2O2 for 1 h and the treatment with 0.1 mM H2O2 for 48 h. Concentrations of chemical are varied from 0 to 0.5 mM.

In Vivo
In vivo	In tumor-bearing hosts, methyl gallate treatment substantially reduces tumor growth and prolonged the survival rate. In contrast, nu/nu mice do not show decreased tumor progression in response to methyl gallate. In addition, in tumor-bearing Treg-depleted mice, tumor growth and the survival rates are not changed by methyl gallate treatment, strongly suggesting that the main therapeutic target of methyl gallate in tumor suppression is related to modulation of the CD4+CD25+ Treg cell functions. In the spleen of tumor-bearing mice, methyl gallate treatment induces a significant decrease in the CD4+CD25+Foxp^3high Treg cell population. Especially, the number of tumor-infiltrating CD25+Foxp^3high Treg cells is significantly lower in methyl gallate-treated mice. methyl gallate can be used to reverse immune suppression and as a potentially useful adjunct for enhancing the efficacy of immune-based cancer therapy^[2].
Animal Research	Animal Models	C57BL/6, Foxp3^EGFPC57BL/6 or nu/nu mice
	Dosages	200 μg
	Administration	i.p.

References

Chemical Information & Solubility

Molecular Weight	184.15	Formula	C₈H₈O₅
CAS No.	99-24-1	SDF	--
Smiles	COC(=O)C1=CC(=C(C(=C1)O)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 36 mg/mL ( (195.49 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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