GSK J4 HCl

Catalog No.S7070

GSK J4 HCl Chemical Structure

Molecular Weight(MW): 453.96

GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.

Size Price Stock Quantity  
In DMSO USD 220 In stock
USD 170 In stock
USD 570 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • DIPG cells were seeded into 96-well plates and treated with panobinostat and GSK-J4 individually or in combination at the indicated concentrations for 72 hr in at least triplicate. Cell viabilities were then assessed using the CelltiterGlo assay relative to 0.1% DMSO control. Data shown as mean ± SD. *indicates the two drugs demonstrate synergy at that condition (i.e. CI < 1).

    Nat Med, 2015, 21(6):555-9. . GSK J4 HCl purchased from Selleck.

    GSK-J4 impaired hair cell regeneration after neomycin damage. (A–D) GSK-J4 reduced the numbers of GFP-positive (green) and FM1-43FX-positive (red) hair cells compared with DMSO-treated controls. Scale bars = 10 μm. (E,F) Quantitative analysis of the number of GFP-positive (E) or FM1-43FX-positive (F) hair cells per neuromast (NM) at different time points in DMSO-treated control and GSK-J4-treated larvae. In the 24-h group, n = 40 neuromasts (20 larvae) per group; in the 48-h group, n = 28 neuromasts (14 larvae) per group. ***p < 0.0001. Bars are mean ± sem. [24-h group: One-way ANOVA; GFP-positive cells: F(2, 117) = 96.94; FM1-43FX-positive cells: F(2, 117) = 114. 48-h group: One-way ANOVA; GFP-positive cells: F(2, 81) = 88.96; FM1-43FX-positive cells: F(2, 81) = 93.85].

    Front Mol Neurosci, 2017. GSK J4 HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Histone Demethylase Inhibitors

Biological Activity

Description GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.
Targets
JMJD3 [1]
(Cell-free assay)
60 nM
In vitro

GSK J4 HCl is an ethyl ester derivative of the JMJD3 selective histone demethylase inhibitor GSK-J1 with an IC50 value greater than 50 μM in vitro. GSK J4 HCl is used to probe the consequences of demethylation of H3K27me3. In human primary macrophages, GSK-J4 inhibits the lipopolysaccharide-induced production of cytokines, including pro-inflammatory tumour necrosis factor (TNF). In addition, GSK-J4 prevents the lipopolysaccharide-induced loss of H3K27me3 associated with the TNF transcription start sites and blocked the recruitment of RNA polymerase II. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CUTLL1 NGC5cYpIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M3KzPFIh|ryP M1;qbGROW09? MUnh[oZm[3S|IHPlcIwh\3Kxd4To MVGyOVE{OjV2OR?=
CUTLL1 MWrBdI9xfG:|aYOgZZN{[Xl? MUKyJO69VQ>? NEPveFdFVVOR MnH2bY5lfWOnczDhdI9xfG:|aYO= M{fkNlI2OTN{NUS5
CUTLL1 NGntWHNHfW6ldHnvckBie3OjeR?= MnnENkDPxE1? M1j2fWROW09? M2LrXolv\HWlZYOgZ4VtdCCleXPs[UBienKnc4S= NVfCNJpWOjVzM{K1OFk>
CUTLL1 Mnf2T4lv[XOnIHHzd4F6 M1LZflYh|ryP NGrKcWxFVVOR Ml;PcIVi\HNidH:gbY5kemWjc3XkJGg{UzJ5bXWz MlTyNlUyOzJ3NEm=
SF7761 NHjYdoRMcW6jc3WgZZN{[Xl? NHzldJI3KM7:TR?= MoKySG1UVw>? NIrndWZqdmO{ZXHz[ZMhUzJ5IH3leIh6dGG2aX;u MUWyOVQxOTZ7Mx?=
SF8628 MXTLbY5ie2ViYYPzZZk> MVO2JO69VQ>? MkT4SG1UVw>? MkHlbY5kemWjc3XzJGszQCCvZYTofYxifGmxbh?= MoLINlU1ODF4OUO=
H3.3 M4PCPGtqdmG|ZTDhd5NigQ>? NULDOYhIPiEQvF2= MmDWSG1UVw>? MYnpcoNz\WG|ZYOgT|I6KG2ndHj5cIF1cW:w M1vr[VI2PDBzNkmz
SF9012 NFjZWFBMcW6jc3WgZZN{[Xl? NFO1bGw3KM7:TR?= NHu5UIRFVVOR NFHzXIhqdmO{ZXHz[ZMhUzNyIH3leIh6dGG2aX;u MYmyOVQxOTZ7Mx?=
SF9402 Ml7ZT4lv[XOnIHHzd4F6 M{m5PVYh|ryP MVjEUXNQ NG\ZW5RqdmO{ZXHz[ZMhUzNzIH3leIh6dGG2aX;u M{fPdVI2PDBzNkmz
SF9427 NGj2OWxMcW6jc3WgZZN{[Xl? MXK2JO69VQ>? Mn[5SG1UVw>? Mn\NbY5kemWjc3XzJGs{OiCvZYTofYxifGmxbh?= NVHtbmdWOjV2MEG2PVM>
human astrocytes MoC2T4lv[XOnIHHzd4F6 MmLqOkDPxE1? NXTlbI9WTE2VTx?= NFnTO|VqdmO{ZXHz[ZMhUzN|IH3leIh6dGG2aX;u Mo\nNlU1ODF4OUO=
SF7761 M{DUUGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 Mn7wOkDPxE1? NIm2dItFVVOR Mnm1bY5pcWKrdIOgT|I4VSCpbHnvcYEh[2WubDDndo94fGh? MnS3NlU1ODF4OUO=
SF8628 M3T6bWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MmLWOkDPxE1? MnH2SG1UVw>? MYXpcohq[mm2czDLNlhOKGeuaX;tZUBk\WyuIHfyc5d1cA>? MV6yOVQxOTZ7Mx?=
H3.3 M3P5N2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NXi1PG1JPiEQvF2= MoHrSG1UVw>? MWTpcohq[mm2czDLNllOKGeuaX;tZUBk\WyuIHfyc5d1cA>? NXv6O5J4OjV2MEG2PVM>
SF9012 NUjLS2lzT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NVfKeZJSPiEQvF2= MkfYSG1UVw>? Mn2ybY5pcWKrdIOgT|MxVSCpbHnvcYEh[2WubDDndo94fGh? NEXjW4QzPTRyMU[5Ny=>
SF9402 NGTVfIxIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M1HBNVYh|ryP MUnEUXNQ NVrwPHlGcW6qaXLpeJMhUzNzTTDncIlwdWFiY3XscEBoem:5dHi= M4jFR|I2PDBzNkmz
SF9427 NVHUb5FsT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MUi2JO69VQ>? NV;KOIlUTE2VTx?= NX\DTpp[cW6qaXLpeJMhUzN{TTDncIlwdWFiY3XscEBoem:5dHi= M3zaUlI2PDBzNkmz
human astrocytes NGnSPIFIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M4HycFYh|ryP NEfsTY5FVVOR NYX3T|g1cW6qaXLpeJMhUzN|TTDncIlwdWFiY3XscEBoem:5dHi= NFzmTVMzPTRyMU[5Ny=>
TG neurons NH;rXnFHfW6ldHnvckBie3OjeR?= NEPFUmY2OCEQvF2= MorJSG1UVw>? NF[4OWVqdmirYnn0d{BJW1ZvMTDy[YFkfGm4YYTpc44h\nKxbTDz[Y5{d3K7IH7leZJwdnN? MkTLNlU2PTJ5MkC=
Th17 MVTGeY5kfGmxbjDhd5NigQ>? NGLWNnQ5OCCwTR?= NXrR[m5rTE2VTx?= MYLpcohq[mm2czDj[YxtKGSrZn\ldoVvfGmjdHnvci=> M4T5clI2QDRyOUmz
β-cells Ml3YSpVv[3Srb36gZZN{[Xl? M2fUU|IxKM7:TR?= M1fJVmROW09? NESzbJNjdHWwdIOgTWZP|rNuIFnsMVHPuixiYX7kJHRPTs7zLXnu[JVk\WRiY3jlcY9scW6nIHflcoUh\XiycnXzd4lwdg>? M1PWT|I3PTB3MUmz
β-cells NVXK[2ozTnWwY4Tpc44h[XO|YYm= MXWyNEDPxE1? NGTiPFFFVVOR M{L5T4lv\HWlZYOg{tIu[2WubDDkfZNnfW6ldHnvci=> Mn7kNlY2ODVzOUO=
ESCs NI[wRlNHfW6ldHnvckBie3OjeR?= M2TwXFEvQOLChdM1US=> NGLQT2hFVVOR MnnZbY5lfWOnczDEUmEh\GGvYXflJIFtd26pIIfpeIgh[WO2aY\heIlwdiCxZjD0bIUhTE6DIHThcYFo\SC{ZYPwc45{\Q>? MXuyOlc2QTF5NR?=
Raw 264.7 M3TpSmZ2dmO2aX;uJIF{e2G7 M1H6[VAvQDF7MvMAieK2VQ>? NXK2UJVtTE2VTx?= MlK5bY5pcWKrdIOgWG5HNc7zIIDyc4R2[3Srb36= NHrWV2IzPjd5NkO2NC=>

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 90 mg/mL warmed (198.25 mM)
Water 90 mg/mL (198.25 mM)
Ethanol 90 mg/mL warmed (198.25 mM)
In vivo Add solvents to the product individually and in order:
2% DMSO+dd H2O
For best results, use promptly after mixing.
10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 453.96
Formula

C24H27N5O2.HCl

CAS No. 1373423-53-0(free base)
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I cannot get a clear solution when I dissolve the compound into water.

  • Answer:

    S7070 GSK J4 HCl can be dissolved in 2% DMSO/dd H2O at 10 mg/mL as a clear solution. Water cannot dissolve it clear directly.

Histone Demethylase Signaling Pathway Map

Tags: buy GSK J4 HCl | GSK J4 HCl supplier | purchase GSK J4 HCl | GSK J4 HCl cost | GSK J4 HCl manufacturer | order GSK J4 HCl | GSK J4 HCl distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID