Home Transmembrane Transporters Potassium Channel inhibitor Doxapram HCl For research use only.

Doxapram HCl

Doxapram HCl inhibits TASK-1, TASK-3, TASK-1/TASK-3 heterodimeric channel function with EC50 of 410 nM, 37 μM, 9 μM, respectively.

Doxapram HCl Chemical Structure

Doxapram HCl Chemical Structure

CAS: 7081-53-0

Purity & Quality Control

Batch: Purity: 99.75%
99.75

Doxapram HCl Related Products

Signaling Pathway

Potassium Channel Signaling Pathways

Potassium Channel Signaling Pathway

Choose Selective Potassium Channel Inhibitors

Biological Activity

Description Doxapram HCl inhibits TASK-1, TASK-3, TASK-1/TASK-3 heterodimeric channel function with EC50 of 410 nM, 37 μM, 9 μM, respectively.
Targets
TASK-1 [1] TASK-1/TASK-3 heterodimeric [1] TASK-3 [1]
410 nM(EC50) 9 μM(EC50) 37 μM(EC50)
In vitro
In vitro Doxapram inhibits both TASK-1 and TASK-3 function in a dose dependent manner. Doxapram inhibition at both hyperpolarized and depolarized potentials, as well as effects independent of extracellular potassium concentration. It is said that the carboxy terminal domain of TASK-1 is important to doxapram inhibition. Doxapram also inhibits TRESK, TASK-2, and TREK-1 but at significantly larger concentrations (EC50s of 240 μM, 460 μM, and >1 mM, respectively). Doxapram has no effect on MAC for halothane. [1]
In Vivo
In vivo Doxapram is an analeptic agent. The respiratory stimulant action is manifested by an increase in tidal volume associated with a slight increase in respiratory rate. A pressor response may result following Doxapram administration. The mean half-life is 3.4 h (range 2.4-4.1h), the mean apparent volume of distribution is 1.5 mg/kg and the whole body clearance is 370 mL/min. Enteric-coated capsules of doxapram base are absorbed rapidly after an initial delay, and the systemic availability is about 60%.[2]
Animal Research Animal Models male Japanese white rabbits
Dosages 0.25 mg/kg/h, 0.50 mg/kg/h
Administration Infusion
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03894189 Unknown status
Weaning Failure
Beni-Suef University
 May 2019 Not Applicable
NCT02421068 Completed
Premature Birth
Sinno H.P. Simons|ZonMw: The Netherlands Organisation for Health Research and Development|Leiden Amsterdam Centre for Drug Research (LACDR)|Dutch Knowledge Centre for Pediatric Pharmacotherapy (NKFK)|Centre for Human Drug Research Netherlands|Radboud University Medical Center|Erasmus Medical Center
 August 2014 --
NCT00389909 Completed
Premature Infants|Apnea
Jean Michel Hascoet|Maternite Regionale Universitaire
 November 2006 Phase 4

Chemical Information & Solubility

Molecular Weight 432.98 Formula

C24H30N2O2.HCl.H2O
CAS No. 7081-53-0 SDF Download Doxapram HCl SDF
Smiles CCN1CC(C(C1=O)(C2=CC=CC=C2)C3=CC=CC=C3)CCN4CCOCC4.O.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 87 mg/mL ( (200.93 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 25 mg/mL

Ethanol : 2 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Doxapram HCl | Doxapram HCl supplier | purchase Doxapram HCl | Doxapram HCl cost | Doxapram HCl manufacturer | order Doxapram HCl | Doxapram HCl distributor