DMOG

Catalog No.S7483

DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolyl hydroxylase.

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DMOG Chemical Structure

DMOG Chemical Structure
Molecular Weight: 175.14

Validation & Quality Control

Quality Control & MSDS

Product Information

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Product Description

Biological Activity

Description DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolyl hydroxylase.
Targets HIF prolyl hydroxylase [1]
In vitro DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]
In vivo DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]
Features

Protocol(Only for Reference)

Cell Assay:

[3]

Cell lines VSMC cells
Concentrations ~100 μM
Incubation Time 72-96 hours
Method

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

Animal Study:

[2]

Animal Models Mice (C57Bl6)
Formulation 8 mg/mouse
Dosages 8 mg in 0.5 ml saline
Administration i.p.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Baader E, et al. Biochem J. 1994, 300 ( Pt 2), 525-530.

[2] Milkiewicz M, et al. J Physiol. 2004, 60(Pt 1), 21-26.

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Chemical Information

Download DMOG SDF
Molecular Weight (MW) 175.14
Formula

C6H9NO5

CAS No. 89464-63-1
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms Dimethyloxalylglycine
Solubility (25°C) * In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name N-(2-Methoxy-2-oxoacetyl)glycine methyl ester

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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