Catalog No.S4107 Synonyms: NSC-141046
Molecular Weight(MW): 473.4
Clofazimine is a rhimophenazine dye, originally developed for the treatment of tuberculosis, it has both antimicrobial and antiinflammatory activity, postulated mechanisms of action include intercalation of clofazimine with bacterial DNA and increasing levels of cellular phospholipase A2.
Purity & Quality Control
Choose Selective Phospholipase (e.g. PLA) Inhibitors
|Description||Clofazimine is a rhimophenazine dye, originally developed for the treatment of tuberculosis, it has both antimicrobial and antiinflammatory activity, postulated mechanisms of action include intercalation of clofazimine with bacterial DNA and increasing levels of cellular phospholipase A2.|
|Features||Intracellular activities of Clofazimine are superior to that of B4154.|
Clofazimine stimulates oxygen consumption and superoxide generation by neutrophils, Clofazimine also causes phospholipase A2 activation in neutrophils, resulting in increased release of lysophosphatidylcholine and arachidonic acid from neutrophil membranes. Clofazimine inhibits mitogen-induced stimulation of peripheral blood mononuclear cells. Clofazimine stabilizes lysosomal membranes in macrophages and inhibits Mycobacterium leprae metabolism in mouse peritoneal macrophages.  Clofazimine (5 mg/mL) causes dose-related enhancement of the activity of phospholipase A2 in S. aureus, according to an increase in the release of 3H-radiolabeled arachidonate and lysophosphatidylethanolamine ([3H]LPE) from bacterial-membrane phospholipids.  Clofazimine inhibits 90% of twenty M. tuberculosis strains with MICs <1.0 μg/mL, to be noted, clofazimine inhibits M. tuberculosis strain 2227 with MICs of 0.06 μg/mL. Clofazimine (1 μg/mL) dose-dependently inhibits activity of J774A.1 macrophages. 
|In vivo||Clofazimine (20 mg/kg) prevents mortality and causes a significant reduction in the numbers of CFU in the lungs and spleens of C57BL/6 mice infected M.tuberculosis H37Rv.  Liposomal Clofazimine (L-CLF) (50 mg/kg) dose-dependently reduces CFU 2 to 3 log units in the spleen, liver, and lungs of acutely infected mice with Mycobacterium tuberculosis Erdman.  Clofazimine (500 μg, bid) results in highest Clofazimine concentrations in spleens and livers of mice whereas Clofazimine concentrations in the lungs are significantly lower. Clofazimine (20 mg/kg) is effective in reducing bacterial loads in the liver, spleen and lungs of C57BL/6 mice experimentally infected with M. avium strain TMC 724. |
-  Arbiser JL, et al. J Am Acad Dermatol, 1995, 32(2 Pt 1), 241-247.
-  Van Rensburg CE, et al. Antimicrob Agents Chemother, 1992, 36(12), 2729-2735.
-  Reddy VM, et al. Antimicrob Agents Chemother, 1996, 40(3), 633-636.
|In vitro||DMSO||5 mg/mL (10.56 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03474198||Recruiting||Tuberculosis Pulmonary||University College London|National University Hospital Singapore|Singapore Clinical Research Institute (SCRI)||March 21 2018||Phase 2|Phase 3|
|NCT02589782||Recruiting||Tuberculosis Multidrug-Resistant|Extensively Drug-Resistant Tuberculosis|Tuberculosis Pulmonary||Medecins Sans Frontieres Netherlands|London School of Hygiene and Tropical Medicine|Global Alliance for TB Drug Development|University College London|Drugs for Neglected Diseases|Swiss Tropical & Public Health Institute|eResearch Technology Inc.|Ministry of Health Republic of Uzbekistan|World Health Organization|Ministry of Health Belarus|TB & HIV Investigative Network (THINK)|Liverpool School of Tropical Medicine||January 2017||Phase 2|Phase 3|
|NCT02754765||Recruiting||Tuberculosis Multidrug-Resistant||Médecins Sans Frontières France|Partners in Health|Harvard Medical School|Epicentre|Institute of Tropical Medicine Belgium|Ministry of Health Kyrgyzstan|National Center for Tuberculosis Problems Kazakhstan|Ministry of Health Lesotho|National Center for Tuberculosis and Lung Diseases Georgia|Socios En Salud Peru||December 2016||Phase 3|
|NCT02409290||Recruiting||MDR-TB||IUATLD Inc|Medical Research Council||April 2016||Phase 3|
|NCT01691534||Completed||Pulmonary Tuberculosis||Global Alliance for TB Drug Development||October 2012||Phase 2|
|NCT01290744||Completed||Borderline Lepromatous Leprosy|Lepromatous Leprosy||Paul Saunderson|Leonard Wood Memorial||August 2010||Phase 4|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.