Name: Hematoxylin
Brand: Selleck Chemicals
SKU: S2384
Rating: 5 (5 reviews)

Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

Hematoxylin Chemical Structure

CAS: 517-28-2

Selleck's Hematoxylin has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 98.79%

98.79

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Biological Activity

Description	Hematoxylin (Hydroxybrazilin) is a compound that forms strongly colored complexes with certain metal ions, notably Fe(III) and Al(III) salts and a kind of stain in histology.

In vitro
In vitro	1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change. 2. Hydrate the samples as follows. i. Transfer the slides through three changes of 100% ethanol for 2 min per change. ii. Transfer to 95% ethanol for 2 min. iii. Transfer to 70% ethanol for 2 min. iv. Rinse the slides in running tap water at room temperature for at least 2 min. 3. Stain the samples in Hematoxylin solution for 3 min. 4. Place the slides under running tap water at room temperature for at least 5 min. 5. Stain the samples in working eosin Y solution for 2 min. 6. Dehydrate the samples as follows. i. Dip the slides in 95% ethanol about 20 times. ii. Transfer to 95% ethanol for 2 min. iii. Transfer through two changes of 100% ethanol for 2 min per change. 7. Clear the samples in three changes of xylene for 2 min per change. 8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT04793230	Completed	Dacryocystitis; Chronic	Al-Azhar University	January 1 2019	--
NCT03483935	Completed	Actinic Keratoses\|Precancerous Skin Lesion	University of Dundee\|Emblation Limited	March 7 2018	Not Applicable

References

[1] Godwin A, J Pharm Clin Sci, 2011, 1, 24-34.

Chemical Information & Solubility

Molecular Weight	302.28	Formula	C₁₆H₁₄O₆
CAS No.	517-28-2	SDF	Download Hematoxylin SDF
Smiles	C1C2=CC(=C(C=C2C3C1(COC4=C3C=CC(=C4O)O)O)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 61 mg/mL ( (201.79 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 61 mg/mL

Ethanol : 61 mg/mL

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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