CCG-1423

Catalog No.S7719

CCG-1423 Chemical Structure

Molecular Weight(MW): 454.75

CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

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2 Customer Reviews

  • Cells were treated with CCG-1423 for 48 h. CCG-1423 blocked the nuclear translocation of MRTF-A.

    Cell Signal, 2017, 31:87-95. CCG-1423 purchased from Selleck.

    CCG-1423 blocked the nuclear translocation of MRTF-A.

    Cellular Signalling, 2017, 31:87-95.. CCG-1423 purchased from Selleck.

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Biological Activity

Description CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.
Targets
RhoA [1]
In vitro

CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. CCG-1423 also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. [1] CCG-1423 in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. [2] In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. [3] CCG-1423, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. [4]

Protocol

Cell Research:[1]
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  • Cell lines: Melanoma lines that overexpress RhoC (A375M2 and SK-Mel-147), low RhoC-expressing lines (A375 and SK-Mel-28), transformed (SW962, PC-3, and SKOV-3) and nontransformed (WI-38) cell lines.
  • Concentrations: 300 nM
  • Incubation Time: 72 hours
  • Method: Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 90 mg/mL (197.91 mM)
Ethanol 4 mg/mL warmed (8.79 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 454.75
Formula

C18H13ClF6N2O3

CAS No. 285986-88-1
Storage powder
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Related Antibodies

Rho Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID