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Carbamoylcholine chloride (Carbachol) AChR agonist

Name: Carbamoylcholine chloride (Carbachol)
Brand: Selleck Chemicals
SKU: S4359
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S4359

Carbamoylcholine chloride (Carbachol, Carbamylcholine chloride, Carbastat, Miostat) is a cholinergic agonist that mimics the effect of acetylcholine on both the muscarinic and nicotinic receptors. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Chemical Structure

Molecular Weight: 182.65

Jump to

Related Targets	Adrenergic Receptor 5-HT Receptor COX Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE) GluR
Other AChR Inhibitors	PNU-120596 Benzethonium Chloride Arecoline HBr Otilonium Bromide Palmatine Lycorine Nitenpyram PNU 282987 HCl Chelidonine Hyoscyamine

Solubility

In vitro
Batch:

Water : 36 mg/mL

Ethanol : 12 mg/mL

DMSO : 5 mg/mL (27.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	182.65	Formula	C₆H₁₅N₂O₂.Cl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	51-83-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Carbamylcholine chloride, Carbastat, Miostat			Smiles	[Cl-].C[N+](C)(C)CCOC(N)=O

Mechanism of Action

In vitro	Carbamoylcholine chloride (Carbachol) causes an immediate increase in short circuit current (Isc) that peaked within 5 min and declined rapidly thereafter in T84 cell monolayers with IC50 of 10 mM, although a small increase in Isc persisted for approximately 30 min. It increases basolateral K+ efflux by activating two different K+ transport pathways on the basolateral membrane in T84 cell monolayers. This compound (100 μM) stimulates a time-dependent increase in tyrosine phosphorylation of a range of proteins in T84 cells. It also stimulates an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase in T84 cells. Additionally, it stimulates tyrosine phosphorylation of the EGF receptor (EGFr) and increases co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr in T84 cells. At a 3.3 nmol dose, it produces a transient increase in NGF mRNA levels present 4-8 hours in hippocampus of rats. It also causes transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend in rats.
In vivo	Infused specifically into the posterior oral pontine reticular nucleus (PnO) of the rat, Carbamoylcholine chloride (Carbachol) (1–10 ng) induces rapid eye movement sleep. At 500 ng, it does not affect REM sleep but enhances wakefulness.
References	[1] https://pubmed.ncbi.nlm.nih.gov/3003156/ [2] https://pubmed.ncbi.nlm.nih.gov/9765228/ [3] https://pubmed.ncbi.nlm.nih.gov/10101239/ [4] https://pubmed.ncbi.nlm.nih.gov/7766858/ [5] https://pubmed.ncbi.nlm.nih.gov/15817635/

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