Name: bisBenzimide H 33258 Trihydrochloride
Brand: Selleck Chemicals
SKU: E2910
Rating: 5 (1 reviews)

bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

bisBenzimide H 33258 Trihydrochloride Chemical Structure

CAS: 23491-45-4

Purity & Quality Control

Batch: E291001 DMSO] 31 mg/mL] false] Water] 23 mg/mL] false] Ethanol] Insoluble] false Purity: 99.60%

99.60

bisBenzimide H 33258 Trihydrochloride Related Products

Biological Activity

Description

bisBenzimide H 33258 Trihydrochloride(Hoechst 33258 trihydrochloride; H 33258 trihydrochloride) is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. It binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity. Therefore, Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. It exhibits anti-tumor activity IC50 of 51.31±4.56 μM in HeLa cells.

References

[1] Wang XJ, et al. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

Chemical Information & Solubility

Molecular Weight	533.88	Formula	C₂₅H₂₄N₆O.3ClH
CAS No.	23491-45-4	SDF	--
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 31 mg/mL ( (58.06 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 23 mg/mL

Ethanol : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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