Batimastat (BB-94)

Catalog No.S7155

Batimastat (BB-94) Chemical Structure

Molecular Weight(MW): 477.64

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.

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3 Customer Reviews

  • To assess cleavage, 2 μg of pro-TNF-α was incubated with either 2 μg ADAM17 or 2 μg MMP9 in the presence or absence of inhibitor. Lanes are as follows: 1. Molecular weight markers, 2. Pro-TNF-α alone, 3. Pro-TNF-α + ADAM17, 4. pro-TNF-α+ ADAM17 + BB-94 (10 μM), 5. Pro-TNF-α + MMP9, 6. Pro-TNF-α + MMP9 + AB0041 (10 μM), 7. Pro-TNF-α+ MMP9 + BB-94 (10 μM), 8. Soluble TNF-α.

    PLoS One, 2015, 10(5): e0127063. Batimastat (BB-94) purchased from Selleck.

    Effect of the broad spectrum hydroxamate inhibitors batimastat and ilomastat on a disintegrin and metalloproteinase with thombospondin type 1 motifs (ADAMTS) 13 and ADAMTS15. (A) While batimastat is a good inhibitor against ADAMTS15, ilomastat has no ability to inhibit the enzyme. ADAMTS15 (20 nM) was pre-incubated with two doses (300 nM or 3 µM) of batimastat and ilomastat prior to the addition of aggrecan substrate G1-IGD-G2. The inhibitory effect of batimastat can be clearly discerned from the undigested 130 kDa G1-IGD-G2 bands. (B) Calculation of the Kiapp value of batimastat by densitometric analysis of the undigested G1-IGD-G2 bands on a 12% SDS-PAGE.

    Biomed Rep, 2016, 4(1):73-78. . Batimastat (BB-94) purchased from Selleck.

  • Calculation of the Kiapp value of batimastat by densitometric analysis of the undigested G1-IGD-G2 bands on a 12% SDS-PAGE.

    Biomed Rep, 2016, 4(1):73-78. Batimastat (BB-94) purchased from Selleck.

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Biological Activity

Description Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
Targets
MMP-1 [1] MMP-2 [1] MMP-9 [1] MMP-7 [1] MMP-3 [1]
3 nM 4 nM 4 nM 6 nM 20 nM
In vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. [1]Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf9 insect cells MnvJSpVv[3Srb36gZZN{[Xl? MWfJcohq[mm2aX;uJI9nKGi3bXHuJI1ifHKreDDt[ZRidGyxcILveIVie2VvMjDlfJBz\XO|ZXSgbY4hW2Z7IHnud4VkfCClZXzsd{whUUN3ME2wMlAyOyEQvF2= MoDmNVU2QDJ2M{[=
Sf9 insect cells MVHGeY5kfGmxbjDhd5NigQ>? M3\KcmlvcGmkaYTpc44hd2ZiaIXtZY4hdWG2cnn4JI1mfGGubH;wdo91\WG|ZT25JIV5eHKnc4Pl[EBqdiCVZkmgbY5{\WO2IHPlcIx{NCCLQ{WwQVAvODJ3IN88US=> M{jLdVE2PTh{NEO2

... Click to View More Cell Line Experimental Data

In vivo Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. [1] In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).[3]Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. [4]

Protocol

Animal Research:[3]
+ Expand
  • Animal Models: Nude mouse
  • Formulation: PBS containing 0.01% Tween 2O
  • Dosages: 30mg/ml
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 96 mg/mL (200.98 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% propylene glycol, 5% Tween 80, 65% D5W
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 477.64
Formula

C23H31N3O4S2

CAS No. 130370-60-4
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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MMP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID