Batimastat (BB-94)

Catalog No.S7155 Batch:S715502

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Technical Data

Formula

C23H31N3O4S2
Molecular Weight 477.64 CAS No. 130370-60-4
Solubility (25°C)* In vitro DMSO 96 mg/mL (200.98 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
6.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.
Targets
MMP-1 [1] MMP-2 [1] MMP-9 [1] MMP-7 [1] MMP-3 [1]
3 nM 4 nM 4 nM 6 nM 20 nM
In vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. [1]

Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. [2]
In vivo

Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. [1]

In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).[3]

Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. [4]

Protocol (from reference)

Kinase Assay:

[1]
  • Enzyme assays

    IC50s are determined for batimastat in vitro against different metalloproteinases in enzyme assays.
Cell Assay:

[1]
  • Cell lines

    B16-BL6 cells

  • Concentrations

    3 nM (MMP-1), 4 nM (MMP-2 & MMP-9), 6 nM (MMP-7) and 20 nM (MMP-3), respectively

  • Incubation Time

    22 h

  • Method

    Cells were treated with different concentrations of batimastat was dissolved in absolute ethanol and were incubated for 22 hr and IC50 was measured.
Animal Study:

[3]
  • Animal Models

    Nude mouse

  • Dosages

    30mg/ml

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Nature, 2017, 549(7673):533-537]

Data from [Data independently produced by , , PLoS One, 2015, 10(5): e0127063]

Data from [Data independently produced by , , Biomed Rep, 2016, 4(1):73-78. ]

Data from [Data independently produced by , , Biomed Rep, 2016, 4(1):73-78]

Selleck's Batimastat (BB-94) has been cited by 32 publications

Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions [ Adv Sci (Weinh), 2023, e2207084.] PubMed: 36737855
FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts [ Cancer Gene Ther, 2023, 30(4):608-621] PubMed: 36494579
Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation [ Sci Adv, 2023, 9(7):eade8939] PubMed: 36791204
Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells [ Nat Commun, 2022, 13(1):5494] PubMed: 36123372
Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate [ Dev Cell, 2022, S1534-5807(22)00040-5] PubMed: 35150612
Systematic analysis of the MAPK signaling network reveals MAP3K-driven control of cell fate [ Cell Syst, 2022, 13(11):885-894.e4] PubMed: 36356576
Complete abrogation of key osteoclast markers with a membrane-anchored tissue inhibitor of metalloproteinase : a novel approach in the prevention of osteoclastogenesis [ Bone Joint Res, 2022, 11(11):763-776] PubMed: 36331083
Aberrant NF-κB activation in odontoblasts orchestrates inflammatory matrix degradation and mineral resorption [ Int J Oral Sci, 2022, 14(1):6] PubMed: 35082271
Bis[2-(Methacryloyloxy) Ethyl] Phosphate as a Primer for Enamel and Dentine [ J Dent Res, 2021, 220345211023477] PubMed: 34238050
GSK-3α/β Activity Negatively Regulates MMP-1/9 Expression to Suppress Mycobacterium tuberculosis Infection [ Front Immunol, 2021, 12:752466] PubMed: 35095838

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.