Catalog No.S2425 Synonyms: Acetovanillone
Molecular Weight(MW): 166.17
Apocynin is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.
2 Customer Reviews
Treatment of rats with apocynin attenuated pancreatic morphological alterations. Representative images from the (A) sham operation (SO) group; (B) severe acute pancreatitis (SAP) group; (C) drug control (APO-CON) group; and (D) apocynin (APO) group (original magnification, ×200). (E) Pancreatic histological scores. Results are presented as the mean ± standard deviation. *P<0.05 vs. SO group; #P<0.05 vs. SAP group.
Mol Med Rep, 2016, 14(4):3525-34.. Apocynin purchased from Selleck.
Purity & Quality Control
Choose Selective NADPH oxidase Inhibitors
|Description||Apocynin is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.|
Apocynin is a naturally occurring methoxy-substituted catechol used as an inhibitor of NADPH-oxidase. Apocynin prevents serine phosphorylation of p47phox and blocks its association with gp91phox, thus blunting NADPH oxidase activation.  Apocynin can decrease the production of superoxide (O(2)(-)) from activated neutrophils and macrophages. Apocynin, after metabolic conversion, inhibits the assembly of NADPH-oxidase that is responsible for reactive oxygen species (ROS) production. 
|In vivo||Apocynin has anti-inflammatory activity in a variety of cell and animal models of inflammation. Apocynin attenuates the TNF-α and IL-1β production and iNOS expression in the lung of carrageenan-treated mice.  Apocynin exerts beneficial effects in a mice model of spinal cord injury. Apocynin reduces (1) the degree of spinal cord damage, (2) neutrophils infiltration, (3) ICAM-1 and P-selectin expression, (4) PAR and nitrotyrosine formation, (5) IκB-α degradation, (6) NF-κB activation, (7) pro-inflammatory cytokines production (TNF-α and IL-1β), (8) apoptosis (TUNEL staining, FAS ligand expression, Bax and Bcl-2 expression) and (9) MAPK activation (P-38 and phospho-JNK).  LD50: Mice 9g/kg (i.g.). |
-  Stolk J,et al. Am J Respir Cell Mol Biol, 1994, 11(1), 95-102.
-  Simons JM, et al. Free Radic Biol Med, 1990, 8(3), 251-258.
-  Stefanska J, et al. Mediators Inflamm, 2008, 106507.
|In vitro||DMSO||33 mg/mL (198.59 mM)|
|Ethanol||33 mg/mL (198.59 mM)|
|Water||slightly soluble or insoluble|
|In vivo||Add solvents individually and in order:
2% DMSO+30% PEG 300+ddH2O
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT01402297||Completed||Chronic Obstructive Pulmonary Disease||Medical Universtity of Lodz|Ministry of Science and Higher Education, Poland||October 2010||Phase 1|
|NCT00992667||Completed||Bronchial Asthma||Medical Universtity of Lodz|Ministry of Science and Higher Education, Poland||June 2008||Phase 1|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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