Name: DPI (Diphenyleneiodonium chloride)
Brand: Selleck Chemicals
SKU: S8639
Rating: 5 (41 reviews)

DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).

DPI (Diphenyleneiodonium chloride) Chemical Structure

CAS: 4673-26-1

Selleck's DPI (Diphenyleneiodonium chloride) has been cited by 41 publications

Purity & Quality Control

Batch: Purity: 99.69%

99.69

DPI (Diphenyleneiodonium chloride) Related Products

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Choose Selective NADPH-oxidase Inhibitors

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MC3T3-E1	Function assay	10 μM	30 min	block ROS generation and NOX expression	31049140
RAW264.7	Function assay	10 μM	4 days	DPI administration inhibited the effect of RANKL on osteoclast differentiation and reduced the number of TRAP-positive multinuclear cells	30942408
VSMC	Function assay	10 μM	6 h	diminished PDGF-BB-evoked VSMC dedifferentiation, proliferation and migration	29175753
HK-2	Function assay			inhibited ROS generation in the TZA-induced necroptosis	28894570
mouse neural precursor cells	Function assay			Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay	17417631
RAW264.7	Function assay		1 hr	Inhibition of LPS-stimulated ROS production in mouse RAW264.7 cells preincubated for 1 hr followed by LPS stimulation after 24 hrs by CMH2DCFDA probe-based fluorescence assay	28384544
Click to View More Cell Line Experimental Data

Biological Activity

Description

DPI (Diphenyleneiodonium chloride) is an inhibitor of NADPH oxidase and also a potent, irreversible, and time-, temperature-dependent iNOS/eNOS inhibitor. Diphenyleneiodonium chloride (DPI) also functions as a TRPA1 activator and selectively inhibits intracellular reactive oxygen species (ROS).

Targets

NADPH oxidase ^[1]

In vitro
In vitro	DPI inhibits the activity of NADPH oxidase, nitric oxide synthase, xanthine oxidase and NADPH cytochrome P450 oxidoreductase^[4]. Femtomolar concentrations of DPI exert potent anti-inflammatory and neuroprotective effects by inhibiting microglial activation through the inhibition of ERK-regulated PHOX activity^[1]. DPI has frequently been used to inhibit ROS production mediated by various flavoenzymes, including NAD(P)H oxidase, quinone oxidoreductase, cytochrome P450 reductase and nitric oxide synthase^[2]. NADPH, NADP+, and 2'5'-ADP blocks the inhibitory action of DPI^[3]. DPI treatment in ARPE-19 cells evoked a dose- and time-dependent growth inhibition, and also induced DNA fragmentation and protein content of the proapoptotic factor Bax. In addition, DPI signiﬁcantly induced the expression and phosphorylation of p53, which induces proapoptotic genes in response to DNA damage or irreparable cell cycle arrest. ROS have been implicated as a key factor in the activation of p53 by many chemotherapeutic drugs^[4].
Cell Research	Cell lines	ARPE-19 cells
	Concentrations	0.1, 1, and 10 μM
	Incubation Time	6, 12, 24, and 48 h
	Method	ARPE-19 cells are plated at 1×10⁶ cells per 60-mm dishes and incubated for 24 h. Cells are cultured in presence or absence of different concentrations of DPI in fresh DMEM/F12 medium supplemented with 10% FBS. After incubation, the cells are trypsinized, washed with phosphate-buffered saline (PBS) and the viable cells were scored by the trypan blue dye exclusion method using a hemocytometer.
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	Nox1 / MMP2 / MMP9		26760964

In Vivo
In vivo	Diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, inhibited the production of pro-inflammatory cytokines, (TNF-α and IL-6), reduced macrophage infiltration and classical polarization, and induced the ROS generation.
Animal Research	Animal Models	Male C57BL/6 mice
	Dosages	1 mg/kg
	Administration	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06061679	Not yet recruiting	COPD (Chronic Obstructive Pulmonary Disease) With Acute Lower Respiratory Infection	Fondazione Policlinico Universitario Agostino Gemelli IRCCS\|University of Florence UNIFI University of Florence Florence Italy Florence	November 2023	Not Applicable
NCT06111664	Recruiting	Mood Disorders	University Hospital Strasbourg France	May 1 2023	--
NCT05706129	Recruiting	Clear Cell Renal Cell Cancer (ccRCC)\|Pancreatic Ductal Adenocarcinoma (PDAC)\|Colorectal Cancer (CRC)	Debiopharm International SA	March 14 2023	Phase 1\|Phase 2
NCT05764681	Not yet recruiting	Cerebral Palsy\|Chronic Post Surgical Pain	Gillette Children''s Specialty Healthcare\|National Institutes of Health (NIH)\|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)\|University of Minnesota	March 2023	--

References

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Chemical Information & Solubility

Molecular Weight	314.55	Formula	C₁₂H₈I.Cl
CAS No.	4673-26-1	SDF	Download DPI (Diphenyleneiodonium chloride) SDF
Smiles	C1=CC=C2C(=C1)C3=CC=CC=C3[I+]2.[Cl-]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 13 mg/mL ( (41.32 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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