Anisomycin

Catalog No.S7409 Synonyms: Flagecidin 

Anisomycin Chemical Structure

Molecular Weight(MW): 265.3

Anisomycin is an antibiotic, which inhibits protein synthesis, and also act as a JNK activator.

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2 Customer Reviews

  • (c) Immunostaining of Cx32, ALB, CPS1, CK18 and ECAD in hESC-Heps induced with SB or anisomycin.

    Sci Rep, 2016, 6:37388. Anisomycin purchased from Selleck.

    Effect of TMZ (100 μmol/l for U87 cells, 50 μmol/l for U251 cells), anisomycin (4 μmol/l), SB203580 (10 μmol/l), TMZ+SB203580 (10 μmol/l) treatment on thephosphorylation of p38 and AQP4 for 24 h in U87 cells and U251 cells, detected by Western blotting. (A) Protein expression of p-p38, p38 and AQP4 in U87 cells with differenttreatments. (B) The ration of p-p38/p38 in U87 cells. (C) The proportion of AQP4 in GAPDH in U87 cells. (D) Protein expression of p-p38, p38 and AQP4 in U251 cells withdifferent treatments. (E) The ration of p-p38/p38 in U251 cells. (F) The proportion of AQP4 in GAPDH in U251 cells. *P< 0.05 versus the control group

    J Cell Biochem, 2017, 118(12):4905-4913. Anisomycin purchased from Selleck.

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Biological Activity

Description Anisomycin is an antibiotic, which inhibits protein synthesis, and also act as a JNK activator.
Targets
JNK [1]
In vitro

Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. Anisomycin (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NFO3PG9EgXSxdH;4bYPDqGG|c3H5 NGjtXJpKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgeI8heHKxZIXj[UBkgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVMh[2WubIOsJGlEPTB;MD6wNkDPxE1? NVHDTZNUOTZyMEWyNVM>
human HeLa cells M3K0bWZ2dmO2aX;uJIF{e2G7 MWKxNEDPxE1? NEi5R45KdmirYnn0bY9vKG:oIITyZY5{dGG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMUCgeW0h[nliM{XTMY1mfGirb37pcoUhdWW2YXLvcIlkKGyjYnXsbY5oKHO2dXT5 NIPNPZMyPTF4NUGzOi=>
mouse RAW264.7 cells M13IS2Z2dmO2aX;uJIF{e2G7 NHXGSlE2KM7:TR?= NG\nXHM{OCCvaX7z NX3BeGwxSWO2aY\heIlwdiCxZjDwN|hOSVCNIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IIDoc5NxcG:{eXzheIlwdiCjdDDUbJIyQDBxVInyNVgzKGG2IEWgeW0h[W[2ZYKgN|AhdWmwczDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nz NXvoWZZFOjN{OUSyPFY>

... Click to View More Cell Line Experimental Data

In vivo Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]

Protocol

Kinase Assay:[2]
+ Expand

JNK phosphorylation:

500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (final concentration 1% v/v). Puromycin is added (final concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the fluorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
Cell Research:[4]
+ Expand
  • Cell lines: Ehrlich ascites carcinoma (EAC) cells
  • Concentrations: 500 ng/mL
  • Incubation Time: 48 h
  • Method: For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 µL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: Male BALB/c mice
  • Formulation: PBS
  • Dosages: 5 mg/kg
  • Administration: peritumorally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 41 mg/mL warmed (154.54 mM)
Ethanol 17 mg/mL warmed (64.07 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+corn oil
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 265.3
Formula

C14H19NO4

CAS No. 22862-76-6
Storage powder
in solvent
Synonyms Flagecidin 

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID