Home Neuronal Signaling COX inhibitor Ampiroxicam For research use only.

Ampiroxicam

Synonyms: CP 65703

Ampiroxicam (CP 65703) is a nonselective cyclooxygenase inhibitor uesd as anti-inflammatory drug.

Ampiroxicam Chemical Structure

Ampiroxicam Chemical Structure

CAS: 99464-64-9

Purity & Quality Control

Batch: S401101 DMSO] 90 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.13%
99.13

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Signaling Pathway

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Biological Activity

Description Ampiroxicam (CP 65703) is a nonselective cyclooxygenase inhibitor uesd as anti-inflammatory drug.
Features Ampiroxicam is a non-acidic ether carbonate prodrug of piroxicam.
Targets
COX [1]
In vitro
In vitro Ampiroxicam (<150 μM) dose-dependently decreases the proliferation of Panc-1 cells. [1] Ampiroxicam (50 μM) results in decreased expression of Sp1, Sp3, Sp4, and VEGFR1 proteins in Panc-1 cells and L3.6pl cells as determined by Western blot analysis. Ampiroxicam (50 μM) results in increased phosphorylation of MAPK1/2 in Panc-1 cells and L3.6pl cells. [2]
Cell Research Cell lines Panc-1 cells
Concentrations 150 μM
Incubation Time 48 hours
Method Panc-1 cells are plated in DME/F12 medium with 5% fetal bovine serum and treated on the next day with vehicle (0.1% DMSO) or various concentrations of Ampiroxicam. Cells are counted at the indicated times with a Coulter Z1 cell counter. Each experiment is done in triplicate, and results are expressed as means, with error bars representing 95% confidence intervals (CIs).
In Vivo
In vivo Ampiroxicam inhibits the stretching response in mice induced by phenylbenzoquinone (PBQ) with maximum protective effect (MPE) of 2 mg/kg. Ampiroxicam inhibits swelling in a dose-responsive manner in the rat foot edema (RFE) assay with ED50 of 28 mg/kg at single oral dose and 7.8 mg/kg at 5 daily oral dose. Ampiroxicam blocks primary and secondary lesion development in rat adjuvant arthritis with ED50 of 2.2 mg/kg and 0.5 mg/kg, respectively. Ampiroxicam (3.2 mg/kg) leads to a plasma concentration of 12 μg/mL at a Tmax of 2 hours for piroxicam derived from ampiroxicam in rats. [3] Ultraviolet-A (UVA)-irradiated 1% Ampiroxicam sensitized in guinea pigs shows positive reaction in the patch testing to UVA-irradiated 1% Ampiroxicam and 1% thiosalicylate (TOS). Concentration of Ampiroxicam is easily reduced by the increase in UVA irradiation doses, as compared with that of piroxicam. [4]
Animal Research Animal Models Rats with adjuvant arthritis
Dosages 3.2 mg/kg
Administration Orally

Chemical Information & Solubility

Molecular Weight 447.46 Formula

C20H21N3O7S
CAS No. 99464-64-9 SDF Download Ampiroxicam SDF
Smiles CCOC(=O)OC(C)OC1=C(N(S(=O)(=O)C2=CC=CC=C21)C)C(=O)NC3=CC=CC=N3
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 90 mg/mL ( (201.13 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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