Catalog No.S1193

Thalidomide Chemical Structure

Molecular Weight(MW): 258.23

Thalidomide was introduced as a sedative drug, immunomodulatory agent and also is investigated for treating symptoms of many cancers. Thalidomide inhibits an E3 ubiquitin ligase, which is a CRBN-DDB1-Cul4A complex.

Size Price Stock Quantity  
USD 80 In stock

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

1 Customer Review

  • Thalidomide and lenalidomide increase the protein level of WT CRBN. Plasmids expressing FS-CRBN WT were transfected into HEK293T cells and then cells were equally divided into each well of a 6-well plate. The cells were treated with thalidomide (100 µM) or lenalidomide (10 µM) for indicated time.

    FASEB Journal, 2015, fj.15-274050. Thalidomide purchased from Selleck.

Purity & Quality Control

Choose Selective TNF-alpha Inhibitors

Click to view more


2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Thalidomide was introduced as a sedative drug, immunomodulatory agent and also is investigated for treating symptoms of many cancers. Thalidomide inhibits an E3 ubiquitin ligase, which is a CRBN-DDB1-Cul4A complex.
TNF-alpha [2]
(Cell-free assay)
In vitro

Thalidomide must be metabolized by the liver to form an epoxide that may be the active teratogenic metabolite. [1] Thalidomide selectively inhibits the production of human monocyte tumor necrosis factor alpha (TNF-alpha) when human monocytes are triggered with lipopolysaccharide and other agonists in culture. [2] Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. [3] Thalidomide acts directly, by inducing apoptosis or G1 growth arrest, in MM cell lines and in patient MM cells that are resistant to melphalan, doxorubicin, and dexamethasone (Dex). Thalidomide enhances the anti-MM activity of Dex and, conversely, are inhibited by interleukin 6. [4] Thalidomide is a potent costimulator of primary human T cells in vitro, synergizing with stimulation via the T cell receptor complex to increase interleukin 2-mediated T cell proliferation and interferon gamma production. Thalidomide also increases the primary CD8+ cytotoxic T cell response induced by allogeneic dendritic cells in the absence of CD4+ T cells. [5]

In vivo Thalidomide (200 mg/kg) results in an inhibition of the area of vascularized cornea of rabbits that ranged from 30% to 51% in three experiments with a median inhibition of 36%. [1]


Solubility (25°C)

In vitro DMSO 52 mg/mL (201.37 mM)
Ethanol 2 mg/mL (7.74 mM)
Water <1 mg/mL
In vivo 30% PEG400+0.5% Tween80+5% propylene glycol 5 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 258.23


CAS No. 50-35-1
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02844309 Recruiting Waldenström Macroglobulinemia Institute of Hematology & Blood Diseases Hospital May 2016 Phase 4
NCT02733380 Recruiting Diffuse Large B-cell Lymphoma Henan Cancer Hospital May 2016 Phase 2
NCT02778893 Recruiting NSCLC Henan Provincial Hospital March 2016 Phase 4
NCT02707484 Not yet recruiting Gastrointestinal Vascular Malformation Shanghai Jiao Tong University School of Medicine March 2016 Phase 3
NCT02748772 Recruiting Colorectal Neoplasms The First Peoples Hospital of Hefei|Anhui Cancer Hospital|Anhui Jimin Cancer Hospital|Simcere Pharmaceutical Co., Ltd January 2016 Phase 3
NCT02301949 Withdrawn Gastrointestinal Bleeding of Unknown Origin|Gastrointestinal Vascular Malformation|Thalidomide Efficiency Zhizheng Ge|Shanghai Jiao Tong University School of Medicine December 2015 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

TNF-alpha Signaling Pathway Map

Related TNF-alpha Products

Tags: buy Thalidomide | Thalidomide ic50 | Thalidomide price | Thalidomide cost | Thalidomide solubility dmso | Thalidomide purchase | Thalidomide manufacturer | Thalidomide research buy | Thalidomide order | Thalidomide mouse | Thalidomide chemical structure | Thalidomide mw | Thalidomide molecular weight | Thalidomide datasheet | Thalidomide supplier | Thalidomide in vitro | Thalidomide cell line | Thalidomide concentration | Thalidomide nmr
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID