Neratinib (HKI-272)

Catalog No.S2150

Neratinib (HKI-272) Chemical Structure

Molecular Weight(MW): 557.04

Neratinib (HKI-272) is a highly selective HER2 and EGFR inhibitor with IC50 of 59 nM and 92 nM in cell-free assays; weakly inhibits KDR and Src, no significant inhibition to Akt, CDK1/2/4, IKK-2, MK-2, PDK1, c-Raf and c-Met. Phase 3.

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3 Customer Reviews

  • HER2 mutations V777L, D769H, V842I, G309A induce gain-of-function over HER2 WT in MCF10A mammary epithelial cells. B, HER2 WT, L755S, and del.755–759 cells were grown in Matrigel in the presence of DMSO vehicle (0.5%), neratinib (0.5  μmol/L) or gefitinib (0.5  μmol/L). Phase contrast images were obtained as in A. C,  MCF10A-HER2 WT or mutants were seeded in soft agar. After 7 days of growth, they were treated with DMSO vehicle (0.5%), lapatinib (0.5 μmol/L) or neratinib (0.5 μmol/L) for an additional week. Error bars represent 95% highest posterior density intervals. *, Significant difference between the HER2 mutant and HER2 WT; #, the effect of inhibitor treatment was significant (95% highest posterior density interval did not contain 0 for both).  D, photomicrographs of the colonies in soft agar on day 12, magnification ×40. 

    Cancer Discov 2013 3, 224-37. Neratinib (HKI-272) purchased from Selleck.

     

    Differential sensitivity of EGFR-mutant glioma and lung cancer cell lines to the irreversible EGFR inhibitors HKI-272 and CI-1033. C, HKI-272 is more potent than CI-1033 in blocking EGFR phosphorylation in SKMG3 cells with EGFR EC mutation. SKMG3 cells were treated with the indicated doses of CI-1033 or HKI-272, and whole lysates were analyzed by immunoblot with the indicated antibodies.

    Cancer Discov 2012 2, 458-471. Neratinib (HKI-272) purchased from Selleck.

  •  

    Differential sensitivity of EGFR-mutant glioma and lung cancer cell lines to the irreversible EGFR inhibitors HKI-272 and CI-1033. A, HKI-272 induces cell death in GBM cells with EGFR EC mutation (SKMG3, SF268) but not EGFR wild-type (WT EGFR) cancer cell lines or astrocytes (NHA). Cell death was assessed by trypan blue exclusion after 5 days of inhibitor treatment. Cells lines in black express wild-type EGFR, whereas those in red contain EGFR EC mutations.

    Cancer Discov 2012 2, 458-471. Neratinib (HKI-272) purchased from Selleck.

Purity & Quality Control

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Neratinib (HKI-272) is a highly selective HER2 and EGFR inhibitor with IC50 of 59 nM and 92 nM in cell-free assays; weakly inhibits KDR and Src, no significant inhibition to Akt, CDK1/2/4, IKK-2, MK-2, PDK1, c-Raf and c-Met. Phase 3.
Targets
HER2 [1]
(Cell-free assay)
EGFR [1]
(Cell-free assay)
KDR [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
PDK-1 [1]
(Cell-free assay)
59 nM 92 nM 800 nM 1.4 μM >5 μM
In vitro

Neratinib weakly inhibits tyrosine kinases KDR and Src with IC50 of 0.8 μM and 1.4 μM, respectively, being 14- and 24-fold less active compared with HER2. Neratinib displays no activity against other serine-threonine kinases such as Akt, cyclin D1/cdk4, cyclin E/cdk2, cyclin B1/cdk1, IKK-2, MK-2, PDK1, c-Raf, and Tpl-2, as well as the tyrosine kinase c-Met. Neratinib selectively inhibits the proliferation of 3T3 cells transfected with the HER2 (3T3/neu), as well as two other HER-2-overexpressing SK-Br-3 and BT474 cells with IC50 values of 2-3 nM, displaying >230-fold potency compared with non-transfected 3T3 cells as well as MDA-MB-435 and SW620 which are EGFR- and HER2-negative. Neratinib also inhibits the proliferation of EGFR-dependent A431 cells with an IC50 of 81 nM. Neratinib reduces HER2 receptor autophosphorylation in BT474 cells with an IC50 of 5 nM, and EGF-dependent phosphorylation of EGFR in A431 cells with IC50 of 3 nM. Blocking of HER-2 by Neratinib results in inhibition of downstream MAPK and Akt pathways with IC50 of 2 nM, more potently than Trastuzumab. Neratinib inhibits the cyclin D1 expression and the phosphorylation of the Rb-susceptibility gene production in BT474 cells with IC50 of 9 nM, leading to G1-S arrest and ultimately decreased cell proliferation. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BT-474 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvqWJdKSzVyPECuNFA2KM7:TR?= MYGyOFAxQTB4NB?=
EFM-192A NGjyUXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fO[GlEPTB:MD6wNFUh|ryP M1TFeVI1ODB7ME[0
HCC1569 M3fTPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXOTWM2ODxyLkCwOUDPxE1? M3joOFI1ODB7ME[0
HCC1954 M4\iXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvWTWM2ODxyLkCwOUDPxE1? M4WwbVI1ODB7ME[0
MDA-MB-175 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zYVmlEPTB:MD6wNFUh|ryP M3HHXVI1ODB7ME[0
MDA-MB-361 MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXKfoNuUUN3MEywMlAxPSEQvF2= MkK2NlQxODlyNkS=
SK-BR-3 M171NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPITWM2ODxyLkCwOUDPxE1? NGTCOJMzPDByOUC2OC=>
UACC-812 NXPkSlFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PKWmlEPTB:MD6wNFUh|ryP MXOyOFAxQTB4NB?=
UACC-893 NWXxUYVYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYL5XHM{UUN3MEywMlAxPSEQvF2= NFrhTnkzPDByOUC2OC=>
SUM-225 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmP4TWM2OD1yLkCxJO69VQ>? M3jjSVI1ODB7ME[0
SUM-190 NXnpd5BrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMEGg{txO MnnjNlQxODlyNkS=
ZR-75-1 NWTHdnhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;SPWZKSzVyPUCuNFMh|ryP MmrCNlQxODlyNkS=
HCC70 M3XNSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzIOGtpUUN3ME2wMlA{KM7:TR?= NHPoTGwzPDByOUC2OC=>
BT-20 NHHtWIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTBwMEeg{txO MYeyOFAxQTB4NB?=
MDA-MB-453 M{T2[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonYTWM2OD1yLkC5JO69VQ>? MmjDNlQxODlyNkS=
HCC1187 MnzlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjFRZY1UUN3ME2wMlExKM7:TR?= MVeyOFAxQTB4NB?=
EFM-19 M3:zPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnG0TWM2OD1yLkGxJO69VQ>? MlPmNlQxODlyNkS=
T-47D NEXtVWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\DTmlEPTB;MD6xOkDPxE1? MX2yOFAxQTB4NB?=
MDA-MB-134 Mor0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTBwMUeg{txO NHXZNlIzPDByOUC2OC=>
HCC38 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYWzVHN3UUN3ME2wMlI2KM7:TR?= NXf1UlhzOjRyMEmwOlQ>
MDA-MB-435 M{\Hfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTvTWM2OD1yLkOzJO69VQ>? M4PpW|I1ODB7ME[0
MDA-MB-468 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;LTWM2OD1yLkOzJO69VQ>? NXvPc3NIOjRyMEmwOlQ>
CAMA-1 M4D0ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHThdFhKSzVyPUCuN|ch|ryP MoSxNlQxODlyNkS=
MDA-MB-436 MmDjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPYTWM2OD1yLkSxJO69VQ>? NE\KS4szPDByOUC2OC=>
MCF-7 MmLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfZTWM2OD1yLkSxJO69VQ>? NWjVWHM2OjRyMEmwOlQ>
MDA-MB-415 M1ewNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwNEKg{txO MorkNlQxODlyNkS=
HCC1806 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI[1TXBKSzVyPUCuOFQh|ryP NV25OpRqOjRyMEmwOlQ>
HCC1395 NF[5XmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXHVVd[UUN3ME2wMlQ6KM7:TR?= Ml7SNlQxODlyNkS=
HCC1937 M4HROmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HFPWlEPTB;MD61NEDPxE1? MUSyOFAxQTB4NB?=
HCC1143 NUnhUmxXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\LTGZKSzVyPUCuOVQh|ryP M1PsclI1ODB7ME[0
UACC-732 NGL3RWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2qyVGlEPTB;MD62OUDPxE1? MUeyOFAxQTB4NB?=
MDA-MB-231 M3nYd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVyxUpM6UUN3ME2xMlAxKM7:TR?= M{TIZVI1ODB7ME[0
MDA-MB-157 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf4cYJKSzVyPUGuNVIh|ryP M1:3SlI1ODB7ME[0
BT-549 M{nkXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLCRXk5UUN3ME2xMlE1KM7:TR?= M3XCeFI1ODB7ME[0
KPL-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPiO25tUUN3ME2xMlg6KM7:TR?= NFrRWIwzPDByOUC2OC=>
CAL-51 NX6yZXJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jX[WlEPTB;MT64PUDPxE1? Mn:5NlQxODlyNkS=
BT474 NUC0N2hLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXLTWM2OD1yLkCwN|I{KMLzIECuNFAxPzVizszN NEHGN5QzOzhzNkK1OC=>
SKBR3 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\4SmlEPTB;MD6wNFc2KMLzIECuNFA2KM7:TR?= M{PRdlI{QDF4MkW0
MDAMB453 NIDDb2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjCV|RKSzVyPUGuOVkhyrFiMD6xO|kh|ryP MoH4NlM5OTZ{NUS=
KB M1;Bcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDNTmtSUUN3ME20MlE{KMLzIECuOFch|ryP MVSyNlQ6OTl|NR?=
KBv200 MkXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH:4fGlKSzVyPU[uNFMhyrFiMD62OEDPxE1? M13GTlIzPDlzOUO1
MCF-7 NGfqe3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXUd|FKSzVyPUOuN|AhyrFiMD60NUDPxE1? M1\CeVIzPDlzOUO1
MCF-7/Adr MlO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTMTWM2OD1iMj64PEDDuSByLkOwJO69VQ>? NFLZR2gzOjR7MUmzOS=>
MCF-7 MnP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrtPYlKSzVyPUOuNFIhyrFiMD6zOEDPxE1? NFi0UGwzOjR7MUmzOS=>
MCF-7/FLV1000 NEO5PFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIKyUlJKSzVyPUeuNFkhyrFiMD63NUDPxE1? M2Hy[|IzPDlzOUO1
HL60 NFrRUVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXrUVVHUUN3ME2yMlI3KMLzIECuNlMh|ryP MUeyNlQ6OTl|NR?=
HL60/Adr MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDrTWM2OD1zLkSyJOKyKDBwMUWg{txO NVXyTJNKOjJ2OUG5N|U>
HEK293/pcDNA3.1 NUPYO|RiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3znPWlEPTB;NT6yPUDDuSByLkWzJO69VQ>? NXvYcm1FOjJ2OUG5N|U>
HEK293/ABCB1 M3P4R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIC0OIRKSzVyPU[uPVEhyrFiMD63NEAh|ryP M1\mXVIzPDlzOUO1
SKBR MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHY[Y4xNjBzLUGwNEBvVQ>? NUDhPItGOy15IHS= NYXPVWczcW6qaXLpeJMh[2WubDDndo94fGhiaX6geIlu\SCjbnSg[I9{\SCmZYDlcoRmdnRibXHucoVz MljXNlE1QDd4MEW=
L858R(EGFR) MYXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NXTER|Jj\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKHSrbXWgZY5lKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= MXuxO|MyOTByMh?=
L858R/T790M(EGFR) Mo[5R4VtdCCYaXHibYxqfHliQYPzZZk> M3rEUYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckB1cW2nIHHu[EBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MWixO|MyOTByMh?=
G776insV_G/C M4flOGNmdGxiVnnhZoltcXS7IFHzd4F6 MnPN[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJJRqdWViYX7kJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? NFGxRVUyPzNzMUCwNi=>
wild-type M2rYRmNmdGxiVnnhZoltcXS7IFHzd4F6 NGnJfpll\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4hfGmvZTDhcoQh\G:|ZTDk[ZBmdmSnboSgcYFvdmW{ Ml\mNVc{OTFyMEK=
A775insYVMA M1P0XWNmdGxiVnnhZoltcXS7IFHzd4F6 MlrJ[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJJRqdWViYX7kJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MXKxO|MyOTByMh?=
G776insV_G/L M1naR2NmdGxiVnnhZoltcXS7IFHzd4F6 NET4Nldl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4hfGmvZTDhcoQh\G:|ZTDk[ZBmdmSnboSgcYFvdmW{ M1rmUFE4OzFzMECy
P780insGSP M4rtdWNmdGxiVnnhZoltcXS7IFHzd4F6 MVrk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6geIlu\SCjbnSg[I9{\SCmZYDlcoRmdnRibXHucoVz MnjPNVc{OTFyMEK=
NCI-H1781 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjqbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5idHnt[UBidmRiZH;z[UBl\XCnbnTlcpQhdWGwbnXy MV[xOlgyQDZzOB?=
HCC827 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrFTFFrcW6qaXLpeJMh[2WubDDndo94fGhiaX6geIlu\SCjbnSg[I9{\SCmZYDlcoRmdnRibXHucoVz MUGxOlgyQDZzOB?=
H3255 NIfEXllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zxTYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIITpcYUh[W6mIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> MnjqNVY5OTh4MUi=
NCI-H1975 NV61fVV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiC2aX3lJIFv\CCmb4PlJIRmeGWwZHXueEBu[W6wZYK= MYSxOlgyQDZzOB?=
A549 MoTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDEenRqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjD0bY1mKGGwZDDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> M{TXUFE3QDF6NkG4
3T3 M3rpNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYGxW4U{UUN3ME23NFAhyrFiN{igcm0> M3jBTVE2OTd|MEC4
3T3/neu NGHKR5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHtTWM2OD1|INMxJFAvOTRibl2= MVyxOVE4OzByOB?=
SK-Br-3 MkToS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvzUZduUUN3ME2yJOKyKDBwMUigcm0> MoLRNVUyPzNyMEi=
BT 474 NV\qTJBNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moi2TWM2OD1{INMxJFAvODZibl2= M3:yOVE2OTd|MEC4
A431 NW\ER|JGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRThzINMxJFkhdk1? NYW1ZWV4OTVzN{OwNFg>
MDA-MB-435 NFXabIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYSxXZdjUUN3ME25OlAhyrFiMU[1JI5O NVrOdotjOTVzN{OwNFg>
SW620 M1PwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\CWWlEPTB;NkmwJOKyKDh2IH7N NEDp[owyPTF5M{CwPC=>

... Click to View More Cell Line Experimental Data

In vivo Oral administration of Neratinib significantly inhibits the growth of 3T3/neu xenografts, with inhibition of 34%, 53%, 98%, and 98% at dose of 10, 20, 40, and 80 mg/kg/day, respectively. Consistent with the inhibition of HER-2 phosphorylation by 84% within 1 hour of administration at 40 mg/kg/day, Neratinib inhibits the growth of BT474 xenografts by 70-82%, 67%, and 93% at dose of 5, 10, and 40 mg/kg/day, respectively. Neratinib is also effective against SK-OV-3 xenografts with inhibition of 31% and 85% at 5 and 60 mg/kg/day, respectively. Neratinib is less potent against EGFR-dependent A431 xenografts than HER-2-dependent tumors, with 32% and 44% inhibition at 5 and 20 mg/kg/day, respectively. Neratinib displays little activity against MCF-7 and MX-1 xenografts expressing low levels of HER-2 and EGFR, with only 28% inhibition at 80 mg/kg/day, suggesting that Neratinib has selective activity for cells expressing HER-2 or EGFR. [1]

Protocol

Kinase Assay:[1]
+ Expand

Cell-free autophosphorylation assay using time-resolved fluorometry:

Neratinib is prepared as 10 mg/mL stocks in DMSO and diluted in 25 mM HEPES (pH 7.5; 0.002 ng/mL-20 μg/mL). Purified recombinant COOH-terminal fragments of HER2 (amino acids 676-1255) or epidermal growth factor receptor (EGFR) (amino acids 645-1186) [diluted in 100 mM HEPES (pH 7.5) and 50% glycerol] is incubated with increasing concentrations of Neratinib in 4 mM HEPES (pH 7.5), 0.4 mM MnCl2, 20 μM sodium vanadate, and 0.2 mM DTT for 15 minutes at room temperature in 96-well ELISA plates. The kinase reaction is initiated by the addition of 40 μM ATP and 20 mM MgCl2 and allowed to proceed for 1 hour at room temperature. Plates are washed, and phosphorylation is detected using Europium-labeled anti-phospho-tyrosine antibodies (15 ng/well). After washing and enhancement steps, signal is detected using a Victor2 fluorescence reader (excitation wavelength 340 nm, emission wavelength 615 nm). The concentration of Neratinib that inhibits receptor phosphorylation by 50% (IC50) is calculated from inhibition curves.
Cell Research:[1]
+ Expand
  • Cell lines: 3T3, 3T3/neu, A431, BT474, SK-Br-3, MDA-MB-435, and SW480
  • Concentrations: Dissolved in DMSO, final concentrations 0.5 ng/mL-5 μg/mL
  • Incubation Time: 2 or 6 days
  • Method: Cells are exposed to various concentrations of Neratinib for 2, or 6 days. Cell proliferation is determined using sulforhodamine B, a protein binding dye. Briefly, cells are fixed with 10% trichloroacetic acid and washed extensively with water. Cells are then stained with 0.1% sulforhodamine B and washed in 5% acetic acid. Protein-associated dye is solubilized in 10 mM Tris, and absorbance is measured at 450 nM. The concentration of Neratinib that inhibits cell proliferation by 50% (IC50) is determined from inhibition curves.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Female athymic (nude) mice implanted s.c. with 3T3/neu, BT474, MCF-7, or SK-OV-3 cells
  • Formulation: Formulated in 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • Dosages: ~80 mg/kg/day
  • Administration: Gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 5 mg/mL (8.97 mM) warming
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 30% PEG400+0.5% Tween80+5% propylene glycol 5 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 557.04
Formula

C30H29ClN6O3

CAS No. 698387-09-6
Storage powder
in solvent
Synonyms N/A

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02977780 Recruiting Glioblastoma Dana-Farber Cancer Institute|Eli Lilly and Company|Celgene|Puma Biotechnology, Inc.|Accelerate Brain Cancer Cure February 9, 2017 Phase 2
NCT02673398 Recruiting HER2 Positive Breast Carcinoma|Recurrent Breast Carcinoma|Stage IV Breast Cancer City of Hope Medical Center|National Cancer Institute (NCI)|Puma Biotechnology, Inc. October 2016 Phase 2
NCT02932280 Recruiting Solid Tumor|Central Nervous System Tumor|Lymphoma|Leukemia Memorial Sloan Kettering Cancer Center|Milton S. Hershey Medical Center|M.D. Anderson Cancer Center|Stanford University|Arkansas Childrens Hospital Research Institute|Alberta Childrens Hospital|Phoenix Childrens Hospital|University of Texas October 2016 Phase 1|Phase 2
NCT02593708 Recruiting Solid Tumor Michelle Melisko|University of California, San Francisco October 2015 Phase 1
NCT02400476 Recruiting Early Stage HER2+ Breast Cancer Puma Biotechnology, Inc. February 2015 Phase 2
NCT02236000 Recruiting Breast Cancer NSABP Foundation Inc|Puma Biotechnology, Inc. August 2014 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID