Home Microbiology Antibiotics inhibitor Methacycline HCl

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Methacycline HCl Antibiotics inhibitor

Cat.No.S2527

Methacycline HCl (Rondomycin) is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.
Methacycline HCl Antibiotics inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 478.88

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Quality Control

Batch: Purity: 99.68%
99.68

Solubility

In vitro
Batch:

DMSO : 95 mg/mL (198.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 1 mg/mL

Ethanol : Insoluble

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In vivo
Batch:

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 478.88 Formula

C22H22N2O8.HCl

 Storage (From the date of receipt)
CAS No. 3963-95-9 Download SDF Storage of Stock Solutions

Synonyms Rondomycin Smiles CN(C)C1C2C(C3C(=C)C4=C(C(=CC=C4)O)C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O.Cl

Mechanism of Action

Targets/IC50/Ki
protein synthesis
In vitro
Methacycline inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline inhibits TGF-β1-induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.
In vivo
Methacycline (100 mg/kg, i.p.) delivered daily beginning 10 days after intratracheal bleomycin improves survival at Day 17. Methacycline attenuates Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA. Methacycline does not attenuate inflammatory cell accumulation or alter TGF-β1-responsive genes in alveolar macrophages. 
References

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