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Spiramycin Antibiotics chemical

Name: Spiramycin
Brand: Selleck Chemicals
SKU: S4082
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4082

Spiramycin (Formacidine) is a 16-membered ring macrolide (antibiotic).

Chemical Structure

Molecular Weight: 843.05

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Related Targets	Integrase Bacterial Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV HCV Protease
Other Antibiotics Inhibitors	G418 Sulfate (Geneticin) Puromycin Nanchangmycin Sitafloxacin Hydrate Fusidine Gamithromycin Thiamphenicol Tildipirosin Nadifloxacin 6-Aminopenicillanic acid

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (118.61 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (5.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.250mg/ml (1.48mM)	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	843.05	Formula	C₄₃H₇₄N₂O₁₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	8025-81-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Formacidine			Smiles	CC1CC=CC=CC(C(CC(C(C(C(CC(=O)O1)O)OC)OC2C(C(C(C(O2)C)OC3CC(C(C(O3)C)O)(C)O)N(C)C)O)CC=O)C)OC4CCC(C(O4)C)N(C)C

Mechanism of Action

In vitro

Spiramycin reduces the hygromycin A protections of nucleotides in 23 S rRNA. This compound, a 16-membered macrolide, inhibits translocation by binding to bacterial 50S ribosomal subunits with an apparent 1:1 stoichiometry. It acts primarily by stimulating the dissociation of peptidyl-tRNA from ribosomes during translocation. This antibiotic at doses sufficient to inhibit protein synthesis in wild-type cells but not sufficient to kill either mutant or wild-type cells at the permissive temperature (30 degrees C). It inhibits protein synthesis by stimulating the dissociation of peptidyl-tRNA from ribosomes. This agent shows dose-related inhibition of the proliferative response of PHA and PWM stimulated human mononuclear leucocytes (MNL). It also induces a decrease in tritiated uridine (3H-UdR) uptake, which suggests that this compound interferes with an early event in the cell cycle. This chemical and, to a lesser extent, erythromycin increases total IL-6 production without affecting IL-1 alpha, IL-1 beta, or tumor necrosis factor alpha production in human monocytes stimulated with lipopolysaccharide. It shows good antimicrobial activity against species of Prevotella, Eubacterium, Peptostreptococcus, Bacteroides and Porphyromonas, and the effect is enhanced by the addition of Metronidazole.

References

[1] https://pubmed.ncbi.nlm.nih.gov/11090288/
[2] https://pubmed.ncbi.nlm.nih.gov/3053566/
[3] https://pubmed.ncbi.nlm.nih.gov/6179465/

[4] https://pubmed.ncbi.nlm.nih.gov/3700286/
[5] https://pubmed.ncbi.nlm.nih.gov/1759822/
[6] https://pubmed.ncbi.nlm.nih.gov/9338486/

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