Catalog No.S1692

Busulfan  Chemical Structure

Molecular Weight(MW): 246.3

Busulfan is a cell cycle non-specific alkylating antineoplastic agent.

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In DMSO USD 130 In stock
USD 97 In stock
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Biological Activity

Description Busulfan is a cell cycle non-specific alkylating antineoplastic agent.
In vitro

Busulfan inhibits the cobblestone area-forming cell frequency but fails to cause a significant increase in apoptosis in hematopoietic stem cell alike cells and progenitors. Busulfan inhibits the hematopoietic function of HSC alike cells and progenitors via an apoptosis-independent mechanism. Busulfan induces bone marrow hematopoietic cell senescence associated with an increased expression of p16Ink4a and p19Arf in a time-dependent manner. [1] Busulfan, an alkylating agent that causes DNA damage by cross-linking DNAs and DNA and proteins, induces senescence in normal human diploid WI38 fibroblasts through the extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (p38 MAPK) cascade independent of the p53-DNA damage pathway. Busulfan induces a transient reduction in GSH but a continuous increase in ROS production. [2] Busulfan-induced hypophosphorylation of Rb prevents apoptosis of spermatogonial stem cells by inhibiting PCNA expression in testicular cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SK-N-SH cells M2L4enBzd2yrZnXyZZRqd25iYYPzZZk> MX:xNE0yODBizszN Mn7xO|IhcA>? NXTxW29sSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT{1PNVOKIHPlcIx{KGG2IEGwJJRwKDFyMDD1UUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5 NIX6NGgzPDhzNEWzNi=>
SiMa cells NYPFRZhEWHKxbHnm[ZJifGmxbjDhd5NigQ>? NFXZN5gyOC1zMECg{txO MYe3NkBp MmTkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVaV3hJINmdGy|IHH0JFExKHSxIEGwNEB2VSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 M2HHbFI1QDF2NUOy
Kelly cells  M{\Cc3Bzd2yrZnXyZZRqd25iYYPzZZk> MkHpNVAuOTByIN88US=> NFzxZlc4OiCq NEfyb3pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFvlcIx6KGOnbHzzJIF1KDFyIITvJFExOCC3TTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7 M4nxOFI1QDF2NUOy
LS cells M13NWHBzd2yrZnXyZZRqd25iYYPzZZk> M1HPc|ExNTFyMDFOwG0> NXy0doU6PzJiaB?= MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyVIHPlcIx{KGG2IEGwJJRwKDFyMDD1UUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5 NGLBPFgzPDhzNEWzNi=>

... Click to View More Cell Line Experimental Data

In vivo Busulfan-treated mice exhibit a marked increase in apoptosis and a decrease in testis weight. Busulfan is administered at the rate of 40 mg/kg body weight to induce a maximal number of apoptotic cells while minimizing the number of necrotic cells. [3] Busulfan conditioning and irradiation results in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis in NOD/SCID mice. [4] Busulfan-transplanted mice has slow and incomplete lymphoid engraftment. Busulfan (20 mg/kg to 100 mg/kg) provides dose-dependent congenic lymphoid reconstitution in mice. [5]


Solubility (25°C)

In vitro DMSO 49 mg/mL (198.94 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 246.3


CAS No. 55-98-1
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03126916 Recruiting Childhood Ganglioneuroblastoma|Childhood Neuroblastoma|INRG Stage L2|INRG Stage M|INRG Stage MS|NMYC Gene Amplification|Recurrent Neuroblastoma Children''s Oncology Group|National Cancer Institute (NCI) May 9 2018 Phase 3
NCT00943800 Active not recruiting Leukemia|Myelodysplastic Syndrome|Multiple Myeloma|Lymphoma University of Chicago October 9 2006 Not Applicable
NCT00547196 Active not recruiting Leukemia|Lymphoma|Myelodysplastic Syndromes City of Hope Medical Center|National Cancer Institute (NCI) August 9 2005 Not Applicable
NCT02512497 Recruiting Cutaneous T-cell Lymphoma|T-Prolymphocytic Leukemia|T-Large Granulocytic Leukemia|T-Lymphoblastic Leukemia/Lymphoma|Peripheral T-Cell Lymphoma Ohio State University Comprehensive Cancer Center|Celgene Corporation December 8 2017 Phase 1
NCT01237951 Completed Myeloma M.D. Anderson Cancer Center November 8 2010 Phase 2
NCT01983969 Completed Advanced Cancers|Lymphoma M.D. Anderson Cancer Center November 7 2013 Phase 1|Phase 2

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    How can I reconstitute the drug for in vivo studies?

  • Answer:

    This compound also can be dissolved in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG and Tween. After they mixed well, dilute with water. S1692 in this formulation is not suitable for long-term storage. Please make the fresh solution each time before use.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID