Busulfan

Catalog No.S1692

Busulfan  Chemical Structure

Molecular Weight(MW): 246.3

Busulfan is a cell cycle non-specific alkylating antineoplastic agent.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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Purity & Quality Control

Choose Selective DNA alkylator Inhibitors

Biological Activity

Description Busulfan is a cell cycle non-specific alkylating antineoplastic agent.
In vitro

Busulfan inhibits the cobblestone area-forming cell frequency but fails to cause a significant increase in apoptosis in hematopoietic stem cell alike cells and progenitors. Busulfan inhibits the hematopoietic function of HSC alike cells and progenitors via an apoptosis-independent mechanism. Busulfan induces bone marrow hematopoietic cell senescence associated with an increased expression of p16Ink4a and p19Arf in a time-dependent manner. [1] Busulfan, an alkylating agent that causes DNA damage by cross-linking DNAs and DNA and proteins, induces senescence in normal human diploid WI38 fibroblasts through the extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (p38 MAPK) cascade independent of the p53-DNA damage pathway. Busulfan induces a transient reduction in GSH but a continuous increase in ROS production. [2] Busulfan-induced hypophosphorylation of Rb prevents apoptosis of spermatogonial stem cells by inhibiting PCNA expression in testicular cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SK-N-SH cells MYXQdo9tcW[ncnH0bY9vKGG|c3H5 MkTCNVAuOTByIN88US=> NYnPZ3M3PzJiaB?= MoexRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSz3OMXNJKGOnbHzzJIF1KDFyIITvJFExOCC3TTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7 M3XQS|I1QDF2NUOy
SiMa cells NVnOZmM3WHKxbHnm[ZJifGmxbjDhd5NigQ>? M1q3cFExNTFyMDFOwG0> NFTJcnk4OiCq MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFOrTXGgZ4VtdHNiYYSgNVAhfG9iMUCwJJVOKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZk> MV[yOFgyPDV|Mh?=
Kelly cells  M1vpTHBzd2yrZnXyZZRqd25iYYPzZZk> NGnWO3UyOC1zMECg{txO Mkj4O|IhcA>? MonmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNZXzsfUBk\WyuczDheEAyOCC2bzCxNFAhfU1iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>? MkDKNlQ5OTR3M{K=
LS cells NES0UWtRem:uaX\ldoF1cW:wIHHzd4F6 MortNVAuOTByIN88US=> NWjtU4E2PzJiaB?= NH;q[FBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzTJINmdGy|IHH0JFExKHSxIEGwNEB2VSCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6 MorqNlQ5OTR3M{K=

... Click to View More Cell Line Experimental Data

In vivo Busulfan-treated mice exhibit a marked increase in apoptosis and a decrease in testis weight. Busulfan is administered at the rate of 40 mg/kg body weight to induce a maximal number of apoptotic cells while minimizing the number of necrotic cells. [3] Busulfan conditioning and irradiation results in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis in NOD/SCID mice. [4] Busulfan-transplanted mice has slow and incomplete lymphoid engraftment. Busulfan (20 mg/kg to 100 mg/kg) provides dose-dependent congenic lymphoid reconstitution in mice. [5]

Protocol

Solubility (25°C)

In vitro DMSO 49 mg/mL (198.94 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 246.3
Formula

C6H14O6S2

CAS No. 55-98-1
Storage powder
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02629120 Recruiting X-Linked Chronic Granulomatious Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) December 3, 2015 Early Phase 1
NCT01176006 Recruiting DOCK8 Deficiency National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) July 28, 2010 Phase 2
NCT02750254 Recruiting Acute Myeloid Leukemia|Myelodysplastic Syndrome|Acute Lymphocytic Leukemia Washington University School of Medicine June 27, 2016 Phase 1|Phase 2
NCT02250937 Recruiting Acute Myeloid Leukemia|Myelodysplastic Syndrome M.D. Anderson Cancer Center October 27, 2014 Phase 2
NCT01861106 Recruiting GATA2|Immunodeficiency|MDS National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) April 26, 2013 Phase 2
NCT01701986 Recruiting Lymphoma M.D. Anderson Cancer Center October 25, 2012 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    How can I reconstitute the drug for in vivo studies?

  • Answer:

    This compound also can be dissolved in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG and Tween. After they mixed well, dilute with water. S1692 in this formulation is not suitable for long-term storage. Please make the fresh solution each time before use.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID