Home Neuronal Signaling COX inhibitor Asaraldehyde For research use only.

Asaraldehyde

Synonyms: Asaronaldehyde

Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.

Asaraldehyde Chemical Structure

Asaraldehyde Chemical Structure

CAS: 4460-86-0

Purity & Quality Control

Batch: S253101 DMSO] 39 mg/mL] false] Ethanol] 16 mg/mL] false] Water] Insoluble] false Purity: 100%
100

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Biological Activity

Description Asaraldehyde (Asaronaldehyde) is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.
Targets
COX-2 [2]
In vitro
In vitro Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, Asaraldehyde is produced in both cultured broths. [1] Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for Asaraldehyde is 17.68 at 100 µg/mL compared to solvent control. [2] Asaraldehyde down-regulates C/EBPβ, C/EBPδ, and C/EBPα. Asaraldehyde suppresses expression of PPARγ1 and PPARγ2. Asaraldehyde also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). [2]
Cell Research Cell lines 3T3-L1 cells
Concentrations 100 μg/mL
Incubation Time 24 hours
Method 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes.

Chemical Information & Solubility

Molecular Weight 196.2 Formula

C10H12O4
CAS No. 4460-86-0 SDF Download Asaraldehyde SDF
Smiles COC1=CC(=C(C=C1C=O)OC)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 39 mg/mL ( (198.77 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble


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In vivo
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