Apigenin

Catalog No.S2262 Synonyms: NSC 83244, LY 080400

Apigenin Chemical Structure

Molecular Weight(MW): 270.24

Apigenin is a potent P450 inhibitor for CYP2C9 with Ki of 2 μM.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 120 In stock
USD 210 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • The effects of apigenin and GLUT-1 AS-ODNs on xenograft apoptosis (the second experiment), observed an under optical microscope (magnification, ×400), and the percentage of apoptotic cells in 100 cells per field was counted and used to calculate the mean apoptosis index (AI). AI, apoptotic index.

    Oncol Rep, 2015, 34(4):1805-14. Apigenin purchased from Selleck.

    Western blotting confirmed that both GLUT-1 (A) and p-Akt (B) were expressed in Hep-2 cells in different apigenin and cisplatin concentration.

    Int J Clin Exp Pathol, 2014, 7(7):3938-3.. Apigenin purchased from Selleck.

Purity & Quality Control

Choose Selective P450 (e.g. CYP17) Inhibitors

Biological Activity

Description Apigenin is a potent P450 inhibitor for CYP2C9 with Ki of 2 μM.
Features Much more potent than kaempferol and myricetin in CT-L inhibition.
Targets
CYP2C9 [5]
2 μM(Ki)
In vitro

Apigenin inhibits PKC by competing with adenosine triphosphate (ATP). Apigenin also reduces the level of TPA-stimulated phosphorylation of cellular proteins and inhibits TPA-induced c-jun and c-fos expression. Apigenin exhibits the reverting effect on the transformed morphology of v-H-ras transformed NIH3T3 cells. [1] Apigenin has been shown to possess anti-mutagenic properties in a setting of nitropyrene-induced genotoxicity in Chinese hamster ovary cells. Apigenin suppresses of LPS-induced cyclooxygenase-2 and nitric oxide synthase-2 activity and expression in mouse macrophages. Apigenin has been reported to inhibit protein kinase C activity, mitogen-activated protein kinase (MAPK), transformation of C3HI mouse embryonic fibroblasts and downstream oncogenes in v-Ha-ras-transformed NIH3T3 cells. Apigenin blocks peroxisome proliferation-regulated kinase (ERK), a MAPK in isolated hepatocytes. Apigenin has further been shown to down-regulate the expression of the Na+/Ca2+-exchanger, a protein important for calcium extrusion in neonatal rat cardiac myocytes. Apigenin induces a reversible G2/M and G0/G1 arrest by inhibiting p34 (cdc2) kinase activity, accompanied by increased p53 protein stability in epidermal cells and fibroblasts. Apigenin is also effective in inhibiting TNFα-induced intracellular adhesion molecule-1 upregulation in cultured human endothelial cells. Apigenin inhibits the expression of HIF-1α and VEGF via the PI3K/Akt/p70S6K1 and HDM2/p53 pathways in human ovarian cancer cells. [2] Apigenin inhibits differentiation by suppressing MAPK signal transduction and reducing API transcription factor level in human keratinocytes. Apigenin also inhibits proliferating of human keratinocytes. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells M13yUmZ2dmO2aX;uJIF{e2G7 NXe3UFg1UW6qaXLpeIlwdiCxZjDDU3gzKHC{b4TlbY4h\XiycnXzd4lwdiCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{whUUN3ME2wMlUh|ryP NVTZTXA4OTZyM{i1N|Y>
human H295R cells NF\HcZZHfW6ldHnvckBie3OjeR?= MWfJcohq[mm2aX;uJI9nKGG{b33heIF{\SCneIDy[ZN{\WRiaX6gbJVu[W5iSEK5OXIh[2WubIOsJGlEPTB;MTFOwG0> MX[xPFc4QDl2NB?=
HEK293 FS cells M1TBN2Z2dmO2aX;uJIF{e2G7 MkXKTY5pcWKrdHnvckBw\iCQT2i0JIV5eHKnc4Pl[EBqdiCKRVuyPVMhTlNiY3XscJMh[XO|ZYPz[YQh[XNiSELPNkBxem:mdXP0bY9vKGK7IFiyU|IwXHm{L1zQU{Bie3OjeTygTWM2OD1zLkGzJO69VQ>? MXOyNFc{OTN3Nx?=
human HeLa cells NX3xfpdITnWwY4Tpc44h[XO|YYm= M{LpOWlvcGmkaYTpc44hd2ZiTWLQNUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiW{PIYWxVSzRidILhcpNxd3K2IHL5JJJieGmmIH\pcJRz[XSrb36gZZN{[XluIFvpQVIvPCEQvF2= M1zMSFE6PzJ3NUe4
human MV4-11 cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= M3TXVFczKGh? NHy5elJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiaHHyZo9zcW6pIF\MWFMhdXW2YYTpc44h[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVIvQDFizszN MmGyNlM1OTFyN{O=
human mast cells MkTiSpVv[3Srb36gZZN{[Xl? Mk\sTY5pcWKrdHnvckBw\iCVWVugbY4hcHWvYX6gcYF{fCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gcYF{fCClZXzsJIRm\3KjboXsZZRqd25uIFXDOVA:OyEQvF2= NWrkfpBVOjF|NUS4NFA>
MDCK cells MmPOSpVv[3Srb36gZZN{[Xl? MVXJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJIlvKE2GQ1ugZ4VtdHNidYPpcochUG:nY3jzeEA{OzN2MjDzeIFqdmmwZzygTWM2OD1|LkGg{txO MX[yNVM2PDhyMB?=
human MDA-kb2 cells M4L3e2Z2dmO2aX;uJIF{e2G7 NHK1[mdCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHHu[JJw\2WwIILlZ4VxfG:{IHnuJIh2dWGwIF3ERU1s[jJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBFUFRvaX7keYNm\CCudXPp[oVz[XOnIHHjeIl3cXS7IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF02NjJizszN NHXoVXoyQTV7MkK0OS=>
MCF-7 MX cells M2jveGZ2dmO2aX;uJIF{e2G7 MX7Jcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJIlvKE2FRj23JG1ZKGOnbHzzJJV{cW6pIFjv[YNpe3RiM{OzOFIhe3SjaX7pcoctKEmFNUC9OU46KM7:TR?= NXjpR3ZiOjF|NUS4NFA>
mouse RAW264.7 cells MXXGeY5kfGmxbjDhd5NigQ>? M1fPNVI1KGh? NHfmemZCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWFNvaX7keYNm\CCwaYTybZRmKGGlY4XteYxifGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQhdWW2aH;kMEBKSzVyPU[uO{DPxE1? M4jK[FE6Pzd6MEi2
human H9 cells MWjGeY5kfGmxbjDhd5NigQ>? MVGzJIRigXN? M4jTSGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gTFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB|IHThfZMh[nlicEK0JIFvfGmpZX6gZ4FxfHW{ZTDhd5NigSxiRVO1NF06KM7:TR?= NFPzNFg5OTV6MU[0
HEK293 cells MXvGeY5kfGmxbjDhd5NigQ>? NVHI[4hnOjRiaB?= MWLB[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPvMYV5eHKnc4Ppcochf2m2aDDHZYw1KHKncH;yeIVzKH[nY4TvdkBi\nSncjCyOEBpenNiYomg[JVidC2udXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwQVI1NjlizszN NXSzNVJvOjR7NUW4PFk>
mouse 26-L5 cells M17OXHBzd2yrZnXyZZRqd25iYYPzZZk> MmflO|IhcA>? Mnj0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SB{Nj3MOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTJ3IN88US=> MknYNVIxOjd5M{m=
human RS4:11 cells M3;WWGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGrWR484OiCq MXHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSV|Q7OTFiY3XscJMhcGG{Yn;ybY5oKHerbHSgeJlx\SCITGSzJIFnfGW{IEeyJIhzeyCkeTD0[ZRz[XqxbHn1cUBj[XOnZDDFfkBEgVSxeDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhT0l3ME2yO{46KM7:TR?= MkDONlM1OTFyN{O=
mouse B16-BL6 cells M{exbXBzd2yrZnXyZZRqd25iYYPzZZk> M1PVNVczKGh? NEH6c2JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFKxOk1DVDZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME2zNU43KM7:TR?= M1T0W|EzODJ5N{O5
human H9 cells M2SwcWN6fG:2b4jpZ4l1gSCjc4PhfS=> Ml7TN{Bl[Xm| Mkn2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFkh[2WubIOgZYZ1\XJiMzDkZZl{NCCLQ{WwQVM2KM7:TR?= MXK4NVU5OTZ2
human HT1080 cells MWPQdo9tcW[ncnH0bY9vKGG|c3H5 NXnmWY1NPzJiaB?= MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJRy=> NInzNYgyOjB{N{ezPS=>
MDCK cells NWXyfFBUS3m2b4TvfIlkcXS7IHHzd4F6 M1zoXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQ1ugZ4VtdHNiYomgUXRVKGG|c3H5MEBESzVyPUO5MlU6KM7:TR?= NGT2fXQyQDZ2MEC0Ni=>
mouse L929 cells NFvkOFdHfW6ldHnvckBie3OjeR?= NH7MWoEyPSCvaX7z MUHQc5RmdnSrYYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iVF7GMYFteGijLXnu[JVk\WRiY4n0c5RwgGmlaYT5JI9nKG2xdYPlJGw6OjliY3XscJMh[XO|ZYPz[YQh[XNic4Xyeol3[WyrdImgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6|IHLl[o9z\SCWTl\hcJBp[SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkh[3K7c4ThcEB3cW:uZYSgd5RicW6rbne= M33hT|kzQDd2MUW=
human THP1 cells NGPaOJdHfW6ldHnvckBie3OjeR?= Ml\hNlAhfU1? M3PSN|EhcA>? M1;TXWlvcGmkaYTpc44hd2ZiTl;YNkBqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IHTve45z\We3bHH0bY9vKG:oIGTQRU1qdmS3Y3XkJGNFOzZibWLORUBmgHC{ZYPzbY9vKGG2IEKwJJVOKGmwY4XiZZRm\CCob4KgNUBpeiCycnnvdkB1dyCWUFGgZ4hidGynbnflJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? Mk\BNlM4QDZ3MkC=
MDA-MB-231 cells MYjGeY5kfGmxbjDhd5NigQ>? MWO1JJVO MlT0TY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? NUO1eFBqOjVzOUC0OlY>

... Click to View More Cell Line Experimental Data

In vivo Apigenin down-regulates production of IL-4 in ovalbumin-immunized BALB/C mice. Apigenin inhibits melanoma lung metastases by impairing interaction of tumor cells with endothelium. Apigenin is shown to cause a significant increase in uterine weight and overall uterine concentration of estrogen receptor (ER)-α in female mice (64) and also suppresses prostate and breast cancer cell growth through estrogen receptor β1. Apigenin suppresses the levels of IGF-I in prostate tumor xenografts and increases levels of IGFBP-3, a binding protein that sequesters IGF-I in vascular circulation. [2] Apigenin (12.5 mg/kg) increases cell proliferation in the dentate gyrus of hippocampus of adult mice. [4]

Protocol

Cell Research:

[5]

+ Expand
  • Cell lines: WI-38, T-24, HT-1376 and PC-3 cells
  • Concentrations: 0, 1, 5, 10, 20, 30, 40, and 50 μg/ml
  • Incubation Time: 24 h
  • Method:

    To measure the effect of apigenin on cell viability, the WI-38, T-24, HT-1376 and PC-3 cells were seeded in 24-well plates (1 × 105 cells/well) for 16-18 h. The cells were then treated with or without various concentrations (0, 1, 5, 10, 20, 30, 40, and 50 μg/ml) of apigenin for 24 h. Each treatment was repeated 3 times. After the exposure period, the medium was removed and followed by washing the cells with PBS. The medium was then changed and incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (5 mg/ml)/well for 4 h. The medium was removed, and formazan was solubilised in isopropanol and measured spectrophotometrically at 563 nm. The percentage of viable cells was estimated by comparing them with the untreated control cells.


    (Only for Reference)
Animal Research:

[6]

+ Expand
  • Animal Models: heterozygous C57BL/TGN TRAMP mice
  • Formulation: 0.5% methyl cellulose and 0.025% Tween 20
  • Dosages: 20 and 50 μg/mouse/day
  • Administration: p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 270.24
Formula

C15H10O5

CAS No. 520-36-5
Storage powder
Synonyms NSC 83244, LY 080400

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

P450 (e.g. CYP17) Signaling Pathway Map

P450 (e.g. CYP17) Inhibitors with Unique Features

Related P450 (e.g. CYP17) Products

Tags: buy Apigenin | Apigenin supplier | purchase Apigenin | Apigenin cost | Apigenin manufacturer | order Apigenin | Apigenin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID