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3-Hydroxybutyric acid (BHB)

Name: 3-Hydroxybutyric acid (BHB)
Brand: Selleck Chemicals
SKU: S1031
Rating: 5 (1 reviews)

Synonyms: 3-hydroxybutanoic acid, β-Hydroxybutyric acid

Catalog No.S1031 Purity: 99.94%

3-Hydroxybutyric acid (BHB) is a small molecule metabolite that is elevated in type I diabetes.,

3-Hydroxybutyric acid (BHB) Chemical Structure

CAS: 300-85-6

Selleck's 3-Hydroxybutyric acid (BHB) has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.94%

99.94

3-Hydroxybutyric acid (BHB) Related Products

Biological Activity

Description	3-Hydroxybutyric acid (BHB) is a small molecule metabolite that is elevated in type I diabetes.,

In vitro
In vitro	3-Hydroxybutyric acid (BHB) suppresses activation of the NLRP3 inflammasome in response to several structurally unrelated NLRP3 activators, without impacting NLRC4, AIM2 or non-canonical caspase-11 inflammasome activation. 3-Hydroxybutyric acid reduces the NLRP3 inflammasome mediated IL-1β and IL-18 production in human monocytes.^[1]
Cell Research	Cell lines	Mouse bone marrow derived macrophages (BMDMs)
	Concentrations	10 mM
	Incubation Time	1 h
	Method	BMDMs are primed with LPS for 4 hours and stimulated with ATP for 1 hour in the presence of various concentrations of 3-Hydroxybutyric acid. Western blot analyses caspase-1 (active subunit p20) and IL-1β (active p17) in the supernatant of BMDMs

In Vivo
In vivo	Exogenous 3‐HB effectively delayed tumor growth in A549 xenograft mice.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05384106	Not yet recruiting	Healthy	Genomatica Inc.	May 25 2022	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	104.10	Formula	C₄H₈O₃
CAS No.	300-85-6	SDF	--
Density	1.126 g/mL at 25 °C
Storage (From the date of receipt)	2 years -20°C(in the dark) liquid	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	2 years -20°C(in the dark) liquid		1 month-20°Cin solvent

In vitro
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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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