Home Metabolism Mitochondrial Metabolism inhibitor NL-1 For research use only.

NL-1

The mitochondrial mitoNEET ligand NL-1 is a mitoNEET inhibitor, which induces a concentration-dependent decrease in cell viability with an IC50 of 47.35 μM and 56.26 μM in REH and REH/Ara-C cells respectively. The docking studies using liver mitochondrial suspensions show the IC50 of NL-1 binding for [3H]-rosiglitazone is 0.9 μM and the Ki for NL-1 site 1 and site 2 was 4.78 and 2.77, respectively.

NL-1 Chemical Structure

NL-1 Chemical Structure

CAS: 188532-26-5

Purity & Quality Control

Batch: S076701 DMSO] 67 mg/mL] false] Ethanol] 8 mg/mL] false] Water] Insoluble] false Purity: 99.9%
99.9

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Biological Activity

Description The mitochondrial mitoNEET ligand NL-1 is a mitoNEET inhibitor, which induces a concentration-dependent decrease in cell viability with an IC50 of 47.35 μM and 56.26 μM in REH and REH/Ara-C cells respectively. The docking studies using liver mitochondrial suspensions show the IC50 of NL-1 binding for [3H]-rosiglitazone is 0.9 μM and the Ki for NL-1 site 1 and site 2 was 4.78 and 2.77, respectively.
Targets
mitoNEET [1]
(in [3H]-rosiglitazone binding test)		 mitoNEET [2]
(in REH cells viability test)		 mitoNEET [2]
(in REH/Ara-C cells viability test)
0.9 μM 47.35 μM 56.26 μM
In vitro
In vitro

NL-1 is a mitoNEET inhibitor with antileukemic effect, which induces a concentration-dependent decrease in cell viability of REH and REH/Ara-C, and impaires the migratory ability of leukemic cells irrespective of the chemoattractant used.[2]
Cell Research Cell lines REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines
Concentrations 10, 20, 30, 40, 50, 60, 70, 80, 100 μM
Incubation Time 72 h
Method

ALL cell lines are plated at 5×104cells/well in a 96-well plate. Following treatment with NL-1, viable cells are quantitated using a Cell Counting Kit-8.
In Vivo
In vivo

NL-1 shows antileukemic activity in an in vivo mouse ALL model.[2]
Animal Research Animal Models Female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 335.46 Formula

C18H25NO3S
CAS No. 188532-26-5 SDF --
Smiles CC(C)(C)C1=CC(=CC(=C1O)C(C)(C)C)CC2C(=O)NC(=O)S2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.72 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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