NL-1

Catalog No.S0767 Batch:S076701

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Technical Data

Formula

C18H25NO3S
Molecular Weight 335.46 CAS No. 188532-26-5
Solubility (25°C)* In vitro DMSO 67 mg/mL (199.72 mM)
Ethanol 8 mg/mL (23.84 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description The mitochondrial mitoNEET ligand NL-1 is a mitoNEET inhibitor, which induces a concentration-dependent decrease in cell viability with an IC50 of 47.35 μM and 56.26 μM in REH and REH/Ara-C cells respectively. The docking studies using liver mitochondrial suspensions show the IC50 of NL-1 binding for [3H]-rosiglitazone is 0.9 μM and the Ki for NL-1 site 1 and site 2 was 4.78 and 2.77, respectively.
Targets
mitoNEET [1]
(in [3H]-rosiglitazone binding test)		 mitoNEET [2]
(in REH cells viability test)		 mitoNEET [2]
(in REH/Ara-C cells viability test)
0.9 μM 47.35 μM 56.26 μM
In vitro

NL-1 is a mitoNEET inhibitor with antileukemic effect, which induces a concentration-dependent decrease in cell viability of REH and REH/Ara-C, and impaires the migratory ability of leukemic cells irrespective of the chemoattractant used.[2]
In vivo

NL-1 shows antileukemic activity in an in vivo mouse ALL model.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines

  • Concentrations

    10, 20, 30, 40, 50, 60, 70, 80, 100 μM

  • Incubation Time

    72 h

  • Method

    ALL cell lines are plated at 5×104cells/well in a 96-well plate. Following treatment with NL-1, viable cells are quantitated using a Cell Counting Kit-8.
Animal Study:

[2]
  • Animal Models

    Female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells

  • Dosages

    10 mg/kg

  • Administration

    i.p.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.